Activating Compound | Comment | Organism | Structure |
---|---|---|---|
squalene and sterol carrier protein | 2fold stimulation of enzyme activity in microsomes, stimulation is lower if microsomes are pretreated with phospholipase A2, acetone powder, 0.05% sodium deoxycholate or 0.2% Triton X-100 | Rattus norvegicus |
General Stability | Organism |
---|---|
extremely labile | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.5 mM, 74% inhibition | Rattus norvegicus | |
4,5-dihydroxy-1,3-benzene-disulfonic acid | trivial name tiron, 0.5 mM, 80% inhibition | Rattus norvegicus | |
bathophenanthroline | 0.5 mM, 71% inhibition | Rattus norvegicus | |
CN- | 0.5 mM, 95% inhibition | Rattus norvegicus | |
dithiothreitol | 1.0 mM, 55% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0357 | - |
5alpha-Cholest-7-en-3beta-ol | reconstituted enzyme system consisting of: cytochrome b5, cytochrome b5 reductase and egg lecithin liposomes | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | Rattus norvegicus | cholesterol biosynthesis | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
multienzyme system | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0079 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, 24 h, 30% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | i.e. lathosterol | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? | |
5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | enzyme is rather a mixed function oxidase which requires O2 and NAD(P)H for activity, than a dehydrogenase | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? | |
5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | NADPH, 40% of activity with NADH | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? | |
5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | cholesterol biosynthesis | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | required as electron carrier | Rattus norvegicus | |
NAD(P)H | - |
Rattus norvegicus |