Application | Comment | Organism |
---|---|---|
medicine | the enzyme can be useful for production of fusaruside, a 10,11-unsaturated immunosuppressive fungal sphingolipid with medical potentials for treating liver injury and colitis | Fusarium graminearum |
synthesis | the enzyme can be useful for production of fusaruside, a 10,11-unsaturated immunosuppressive fungal sphingolipid with medical potentials for treating liver injury and colitis, DELTA 10(E)-sphingolipid desaturase as a new regiospecific biocatalyst | Fusarium graminearum |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged wild-type and mutant enzymes in Pichia pastoris strain GS115 | Fusarium graminearum |
Protein Variants | Comment | Organism |
---|---|---|
D281A | site-directed mutagenesis, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
H280A | site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
H284A | site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
H317A | site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
H320A | site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
H321A | site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
H504A | site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
H505A | site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive | Fusarium graminearum |
additional information | knockout of the DELTA10(E)-SD gene abolishes fusaruside production, overview | Fusarium graminearum |
V502A | site-directed mutagenesis, the expressed mutant enzyme is soluble and shows slightly reduced catalytic activity compared to the wild-type enzyme | Fusarium graminearum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics | Fusarium graminearum | |
0.00206 | - |
cerebroside B | recombinant wild-type enzyme, pH 8.0, 28°C | Fusarium graminearum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Fusarium graminearum | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ | Fusarium graminearum | a (4E,8E)-sphinga-4,8-dienine ceramide | fusaruside + 2 ferricytochrome b5 + 2 H2O | - |
? | |
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ | Fusarium graminearum CBS123657 | a (4E,8E)-sphinga-4,8-dienine ceramide | fusaruside + 2 ferricytochrome b5 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium graminearum | A0A016Q182 | - |
- |
Fusarium graminearum CBS123657 | A0A016Q182 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Pichia pastoris strain GS115 by nickel affinity chromatography | Fusarium graminearum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Fusarium graminearum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ | a (4E,8E)-sphinga-4,8-dienine ceramide | Fusarium graminearum | fusaruside + 2 ferricytochrome b5 + 2 H2O | - |
? | |
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ | a (4E,8E)-sphinga-4,8-dienine ceramide, high substrate | Fusarium graminearum | fusaruside + 2 ferricytochrome b5 + 2 H2O | - |
? | |
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ | a (4E,8E)-sphinga-4,8-dienine ceramide | Fusarium graminearum CBS123657 | fusaruside + 2 ferricytochrome b5 + 2 H2O | - |
? | |
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ | a (4E,8E)-sphinga-4,8-dienine ceramide, high substrate | Fusarium graminearum CBS123657 | fusaruside + 2 ferricytochrome b5 + 2 H2O | - |
? | |
additional information | LC-MS screenings of sphingolipid-enriched fractions and in vitro enzymatic synthesis of fusaruside by recombinant DELTA 10(E)-SD. The enzyme shows no reactivity towards 2-methylbutene-bearing molecules including citral, isochromophilone VI, nerolidol, phytolorvitamin D1 and cerebroside B derived ceramide. The presence of the C9-methyl group is essential for the sphingolipid DELTA 10-desaturase activity, mutational analysis | Fusarium graminearum | ? | - |
? | |
additional information | LC-MS screenings of sphingolipid-enriched fractions and in vitro enzymatic synthesis of fusaruside by recombinant DELTA 10(E)-SD. The enzyme shows no reactivity towards 2-methylbutene-bearing molecules including citral, isochromophilone VI, nerolidol, phytolorvitamin D1 and cerebroside B derived ceramide. The presence of the C9-methyl group is essential for the sphingolipid DELTA 10-desaturase activity, mutational analysis | Fusarium graminearum CBS123657 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA 10(E)-SD | - |
Fusarium graminearum |
DELTA 10(E)-sphingolipid desaturase | - |
Fusarium graminearum |
sphingolipid 10,11-desaturase | - |
Fusarium graminearum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Fusarium graminearum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Fusarium graminearum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Fusarium graminearum | |
additional information | the enzyme assay is performed with 2 mM NADH | Fusarium graminearum |
General Information | Comment | Organism |
---|---|---|
metabolism | the fusaruside biosynthetic pathway involves the enzyme, overview | Fusarium graminearum |
physiological function | the enzyme catalyzes the regiospecific biosynthesis of fusaruside, a 10,11-unsaturated immunosuppressive fungal sphingolipid through desaturation of cerebroside B, an abundant fungal sphingolipid. The conserved histidines of DELTA 10(E)-SD are essential for the 10,11-desaturation catalysis, which is also preconditioned by the C9-methylation of the substrate. Enzyme DELTA 10(E)-SD improves survival of fungal strains in chill and salinity | Fusarium graminearum |