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Literature summary for 1.14.19.18 extracted from

  • Li, S.F.; Song, L.Y.; Zhang, G.J.; Yin, W.B.; Chen, Y.H.; Wang, R.R.; Hu, Z.M.
    Newly identified essential amino acid residues affecting DELTA8-sphingolipid desaturase activity revealed by site-directed mutagenesis (2011), Biochem. Biophys. Res. Commun., 416, 165-171.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in Saccharomyces cerevisae strain INVScI Brassica rapa subsp. oleifera

Protein Variants

Protein Variants Comment Organism
D208R inactive Brassica rapa subsp. oleifera
D210R inactive Brassica rapa subsp. oleifera
F59R the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
G28R the mutant shows slightly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
G368R inactive Brassica rapa subsp. oleifera
G52R the mutant shows wild type activity Brassica rapa subsp. oleifera
H63R inactive Brassica rapa subsp. oleifera
L369A the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
L71R the mutant shows wild type activity Brassica rapa subsp. oleifera
N203R inactive Brassica rapa subsp. oleifera
Q372H the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
T56R the mutant shows slightly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
W190A the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
W345A the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
Y31A the mutant shows slightly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Brassica rapa subsp. oleifera 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+ Brassica rapa subsp. oleifera
-
(4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Brassica rapa subsp. oleifera G9I7G1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+
-
Brassica rapa subsp. oleifera (4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O
-
?

Synonyms

Synonyms Comment Organism
DELTA8-sphingolipid desaturase
-
Brassica rapa subsp. oleifera

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
Brassica rapa subsp. oleifera