Application | Comment | Organism |
---|---|---|
pharmacology | the cells capacity to biosynthesize dihydroceramides must be taken into account in proautophagic Des1 inhibitors-including therapies | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
celecoxib | CCX | Homo sapiens | |
fenretinide | - |
Homo sapiens | |
additional information | in T98G and U87MG glioblastoma cell lines different Des1 inhibitory pro-autophagic compounds (DIPACS) exhibiting different cytotoxicities (CCX, PXD, resveratrol, gamma-tocotrienol and XM462), trigger autophagy via different pathways, both dihydroceramide-dependent and independent pathways | Homo sapiens | |
phenoxodiol | PXD | Homo sapiens | |
resveratrol | - |
Homo sapiens | |
tetrahydrocannabinol | - |
Homo sapiens | |
XM462 | an active-site directed Des1 inhibitor | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetracosanoyl-dihydroceramide + reduced acceptor + O2 + 2 H+ | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
glioblastoma cell | - |
Homo sapiens | - |
T-98G cell | - |
Homo sapiens | - |
U-87DND cell | - |
Homo sapiens | - |
U-87MG cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphinganine + reduced acceptor + O2 + 2 H+ | - |
Homo sapiens | ? | - |
? | |
tetracosanoyl-dihydroceramide + reduced acceptor + O2 + 2 H+ | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dihydroceramide desaturase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | gamma-tocotrienol causes downregulation of Des1 expression but is not inhibitory for the enzyme activity | down |
General Information | Comment | Organism |
---|---|---|
malfunction | dihydroceramide desaturase 1 inhibitors activate autophagy via both dihydroceramide-dependent and independent pathways and the balance between the two pathways influences the final cell fate. Enzyme inhibitors celecoxib, resveratrol, phenoxodiol, and gamma-tocotrienol, but not gamma-tocopherol at 0.05 mM, promote an increase in dihydroceramide, dihydrosphingomyelins, and lactosyldihydroceramides in U-87MG and T-98G cell lines. Similar results are found with the active site-directed Des1 inhibitor XM462 | Homo sapiens |