BRENDA - Enzyme Database
show all sequences of 1.14.19.16

DELTA12-Oleate desaturase-related enzymes associated with formation of conjugated trans-DELTA11, cis-DELTA13 double bonds

Iwabuchi, M.; Kohno-Murase, J.; Imamura, J.; J. Biol. Chem. 278, 4603-4610 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Arabidopsis thaliana seeds and in Saccharomyces cerevisiae D452-2 cells
Punica granatum
expressed in Arabidopsis thaliana seeds and in Saccharomyces cerevisiae D452-2 cells
Trichosanthes kirilowii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Punica granatum
-
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Trichosanthes kirilowii
-
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Punica granatum
Q84UB8
-
-
Trichosanthes kirilowii
Q84UC0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Punica granatum
-
seed
-
Trichosanthes kirilowii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(9Z,12Z)-hexadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
?
-
-
-
?
(9Z,12Z)-hexadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
?
-
-
-
?
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
?
-
-
-
?
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
?
-
-
-
?
(9Z,12Z,15Z)-octadec-9,12,15-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
?
-
-
-
?
(9Z,12Z,15Z)-octadec-9,12,15-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
?
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
additional information
no activity with oleic acid and palmitoleic acid
734143
Punica granatum
?
-
-
-
-
additional information
no activity with oleic acid and palmitoleic acid
734143
Trichosanthes kirilowii
?
-
-
-
-
additional information
the enzyme has both conjugase and DELTA12-oleate desaturase activities
734143
Punica granatum
?
-
-
-
-
additional information
the enzyme has both conjugase and DELTA12-oleate desaturase activities
734143
Trichosanthes kirilowii
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Punica granatum
cytochrome b5
-
Trichosanthes kirilowii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Arabidopsis thaliana seeds and in Saccharomyces cerevisiae D452-2 cells
Punica granatum
expressed in Arabidopsis thaliana seeds and in Saccharomyces cerevisiae D452-2 cells
Trichosanthes kirilowii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Punica granatum
cytochrome b5
-
Trichosanthes kirilowii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Punica granatum
-
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
Trichosanthes kirilowii
-
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Punica granatum
-
seed
-
Trichosanthes kirilowii
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(9Z,12Z)-hexadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
?
-
-
-
?
(9Z,12Z)-hexadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
?
-
-
-
?
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
?
-
-
-
?
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
?
-
-
-
?
(9Z,12Z,15Z)-octadec-9,12,15-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
?
-
-
-
?
(9Z,12Z,15Z)-octadec-9,12,15-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
?
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Punica granatum
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
-
734143
Trichosanthes kirilowii
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
-
-
?
additional information
no activity with oleic acid and palmitoleic acid
734143
Punica granatum
?
-
-
-
-
additional information
no activity with oleic acid and palmitoleic acid
734143
Trichosanthes kirilowii
?
-
-
-
-
additional information
the enzyme has both conjugase and DELTA12-oleate desaturase activities
734143
Punica granatum
?
-
-
-
-
additional information
the enzyme has both conjugase and DELTA12-oleate desaturase activities
734143
Trichosanthes kirilowii
?
-
-
-
-
Other publictions for EC 1.14.19.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734143
Iwabuchi
DELTA12-Oleate desaturase-rela ...
Punica granatum, Trichosanthes kirilowii
J. Biol. Chem.
278
4603-4610
2003
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
2
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733773
Hornung
Formation of conjugated DELTA1 ...
Punica granatum
Eur. J. Biochem.
269
4852-4859
2002
-
-
1
-
-
-
-
-
-
-
1
1
-
3
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-