BRENDA - Enzyme Database
show all sequences of 1.14.19.14

Identification and analysis of a gene from Calendula officinalis encoding a fatty acid conjugase

Qiu, X.; Reed, D.; Hong, H.; MacKenzie, S.; Covello, P.; Plant Physiol. 125, 847-855 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Saccharomyces cerevisiae strain AMY-2alpha
Calendula officinalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43600
-
x * 43600, calculated from amino acid sequence
Calendula officinalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + reduced acceptor + O2
Calendula officinalis
best substrate
(8E,10E,12Z)-octadec-8,10,12-trienoly-[glycerolipid] + acceptor + H2O
i.e. calendic acid
-
?
Organism
Organism
UniProt
Commentary
Textmining
Calendula officinalis
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Calendula officinalis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(9Z)-hexadec-9-enoyl-[glycerolipid] + reduced acceptor + O2
weak activity
734947
Calendula officinalis
(8Z,10Z)-hexadec-8,10-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
(9Z)-octadec-9-enoyl-[glycerolipid] + reduced acceptor + O2
weak activity
734947
Calendula officinalis
(8Z,10Z)-octadec-8,10-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + reduced acceptor + O2
best substrate
734947
Calendula officinalis
(8E,10E,12Z)-octadec-8,10,12-trienoly-[glycerolipid] + acceptor + H2O
i.e. calendic acid
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 43600, calculated from amino acid sequence
Calendula officinalis
Synonyms
Synonyms
Commentary
Organism
Fac2
-
Calendula officinalis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain AMY-2alpha
Calendula officinalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43600
-
x * 43600, calculated from amino acid sequence
Calendula officinalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + reduced acceptor + O2
Calendula officinalis
best substrate
(8E,10E,12Z)-octadec-8,10,12-trienoly-[glycerolipid] + acceptor + H2O
i.e. calendic acid
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Calendula officinalis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(9Z)-hexadec-9-enoyl-[glycerolipid] + reduced acceptor + O2
weak activity
734947
Calendula officinalis
(8Z,10Z)-hexadec-8,10-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
(9Z)-octadec-9-enoyl-[glycerolipid] + reduced acceptor + O2
weak activity
734947
Calendula officinalis
(8Z,10Z)-octadec-8,10-dienoyl-[glycerolipid] + acceptor + H2O
-
-
-
?
(9Z,12Z)-octadec-9,12-dienoyl-[glycerolipid] + reduced acceptor + O2
best substrate
734947
Calendula officinalis
(8E,10E,12Z)-octadec-8,10,12-trienoly-[glycerolipid] + acceptor + H2O
i.e. calendic acid
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43600, calculated from amino acid sequence
Calendula officinalis
Other publictions for EC 1.14.19.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732650
Dyer
Molecular analysis of a bifunc ...
Vernicia fordii
Plant Physiol.
130
2027-2038
2002
-
-
1
-
-
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-
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1
-
-
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1
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2
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6
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1
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1
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1
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2
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6
-
-
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-
-
-
-
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1
1
-
-
-
734136
Cahoon
Formation of conjugated DELTA8 ...
Calendula officinalis
J. Biol. Chem.
276
2637-2643
2001
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-
1
-
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-
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2
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2
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1
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2
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2
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2
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2
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2
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
734947
Qiu
Identification and analysis of ...
Calendula officinalis
Plant Physiol.
125
847-855
2001
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
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3
1
1
-
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1
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-
1
1
-
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-
-
1
-
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3
1
-
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-
-
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