BRENDA - Enzyme Database show
show all sequences of 1.14.19.13

Identification and characterization of a novel bifunctional DELTA12/DELTA15-fatty acid desaturase gene from Rhodosporidium kratochvilovae

Cui, J.; He, S.; Ji, X.; Lin, L.; Wei, Y.; Zhang, Q.; Biotechnol. Lett. 38, 1155-1164 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae
Rhodotorula kratochvilovae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Rhodotorula kratochvilovae
the enzyme produces linoleic acid and alpha-linolenic acid, overview
?
-
-
-
additional information
Rhodotorula kratochvilovae YM25235
the enzyme produces linoleic acid and alpha-linolenic acid, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodotorula kratochvilovae
A0A024B710
isolated from Chenghai Lake in Yunnan, China
-
Rhodotorula kratochvilovae YM25235
A0A024B710
isolated from Chenghai Lake in Yunnan, China
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2
-
744576
Rhodotorula kratochvilovae
(9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O
-
-
-
?
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2
-
744576
Rhodotorula kratochvilovae YM25235
(9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O
-
-
-
?
additional information
the enzyme produces linoleic acid and alpha-linolenic acid, overview
744576
Rhodotorula kratochvilovae
?
-
-
-
-
additional information
absence of desaturation activity of RKD12 for C16 substrates
744576
Rhodotorula kratochvilovae
?
-
-
-
-
additional information
the enzyme produces linoleic acid and alpha-linolenic acid, overview
744576
Rhodotorula kratochvilovae YM25235
?
-
-
-
-
additional information
absence of desaturation activity of RKD12 for C16 substrates
744576
Rhodotorula kratochvilovae YM25235
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 50600, about, sequence calculation
Rhodotorula kratochvilovae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae
Rhodotorula kratochvilovae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Rhodotorula kratochvilovae
the enzyme produces linoleic acid and alpha-linolenic acid, overview
?
-
-
-
additional information
Rhodotorula kratochvilovae YM25235
the enzyme produces linoleic acid and alpha-linolenic acid, overview
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2
-
744576
Rhodotorula kratochvilovae
(9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O
-
-
-
?
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2
-
744576
Rhodotorula kratochvilovae YM25235
(9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O
-
-
-
?
additional information
the enzyme produces linoleic acid and alpha-linolenic acid, overview
744576
Rhodotorula kratochvilovae
?
-
-
-
-
additional information
absence of desaturation activity of RKD12 for C16 substrates
744576
Rhodotorula kratochvilovae
?
-
-
-
-
additional information
the enzyme produces linoleic acid and alpha-linolenic acid, overview
744576
Rhodotorula kratochvilovae YM25235
?
-
-
-
-
additional information
absence of desaturation activity of RKD12 for C16 substrates
744576
Rhodotorula kratochvilovae YM25235
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 50600, about, sequence calculation
Rhodotorula kratochvilovae
Expression
Organism
Commentary
Expression
Rhodotorula kratochvilovae
a culture temperature downshift from 28C to 15C induces the enzyme
up
General Information
General Information
Commentary
Organism
evolution
bifunctional DELTA12/DELTA15-desaturases are widely found in fungi species belonging to Ascomycota and Basidiomycota. Enzyme RKD12 shares higher homology to the Cop-odeA, it only exhibits DELTA12 and DELTA15 desaturation activity on C18 substrates without preference
Rhodotorula kratochvilovae
physiological function
for better adaptation to low temperatures, Rhodosporidium kratochvilovae YM25235 may have evolved some adaptative mechanisms that might include the alteration of unsaturation levels of fatty acids in membrane lipids involving the enzyme
Rhodotorula kratochvilovae
General Information (protein specific)
General Information
Commentary
Organism
evolution
bifunctional DELTA12/DELTA15-desaturases are widely found in fungi species belonging to Ascomycota and Basidiomycota. Enzyme RKD12 shares higher homology to the Cop-odeA, it only exhibits DELTA12 and DELTA15 desaturation activity on C18 substrates without preference
Rhodotorula kratochvilovae
physiological function
for better adaptation to low temperatures, Rhodosporidium kratochvilovae YM25235 may have evolved some adaptative mechanisms that might include the alteration of unsaturation levels of fatty acids in membrane lipids involving the enzyme
Rhodotorula kratochvilovae
Expression (protein specific)
Organism
Commentary
Expression
Rhodotorula kratochvilovae
a culture temperature downshift from 28C to 15C induces the enzyme
up
Other publictions for EC 1.14.19.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744576
Cui
Identification and characteri ...
Rhodotorula kratochvilovae, Rhodotorula kratochvilovae YM25235
Biotechnol. Lett.
38
1155-1164
2016
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