Cloned (Comment) | Organism |
---|---|
gene scd1, quantitative real-time PCR expression analysis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2-(1-(3,4,5-trichlorobenzyl)-1H-1,2,3-triazol-4-yl)imidazo[1,2-a]pyridin-7-yl)methanol | - |
Homo sapiens | |
(3(2)R,3(5)S,5S)-5-(tert-butyl)-N-((1R,2S)-1-((cyclopropylsulfonyl)carbamoyl)-2-vinylcyclopropyl)-4,7-dioxo-2,8-dioxa-6-aza-1(1,7)-isoquinolina-3(2,1)-pyrrolidinacyclotridecaphane-3(5)-carboxamide | - |
Homo sapiens | |
(5-(6-(3-(2-(trifluoromethyl)phenoxy)azetidin-1-yl)pyridazin-3-yl)-1,3,4-oxadiazol-2-yl)methanol | - |
Homo sapiens | |
(5-(6-(4-(2-(trifluoromethyl)phenoxy)piperidin-1-yl)pyridazin-3-yl)-1,3,4-oxadiazol-2-yl)methanol | - |
Homo sapiens | |
(5-(6-(4-(2-bromo-5-fluorophenoxy)piperidin-1-yl)pyridazin-3-yl)pyridin-3-yl)methanol | - |
Homo sapiens | |
5-(4-(2-bromo-5-fluorophenoxy)piperidin-1-yl)thiazolo[5,4-d]pyrimidin-2-amine | - |
Homo sapiens | |
additional information | no inhibition by NS3 protease inhibitor B. Palmitoleic acid or oleic acid treatment rescues SCD inhibitors' antiviral effect | Homo sapiens | |
N-(2-(7-((4-chloro-3-(trifluoromethyl)benzyl)amino)-3-(4-methoxyphenyl)-2-oxoquinoxalin-1(2H)-yl)ethyl)acetamide | - |
Homo sapiens | |
[3-(2-[4-[2-(trifluoromethyl)phenoxy]piperidin-1-yl]-1,3-thiazol-5-yl)-1,2,4-oxadiazol-5-yl]methanol | EC50 value is 62 nM. SCD-1 inhibition with the inhibitor increases susceptibility of hepatitis C virus RNA to exogenously added nucleases in digitonin permeabilized cells. Low dose treatment with the inhibitor decreases the number of membranous webs and alters their structure while having no effect on lipid droplets in Huh7-SGR cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Homo sapiens | - |
palmitoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Homo sapiens | - |
oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00767 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
hepatoma cell | - |
Homo sapiens | - |
Huh-7 cell | harboring the pFKI389neo/NS3-39/5.1 subgenomic replicon (Huh7 SGR, genotype 1b) | Homo sapiens | - |
Huh-7.5 cell | stably expressing the full-length HCV genotype 1b replicon with a S2204I adaptive mutation in NS5A (Huh7.5 FGR) | Homo sapiens | - |
liver | high expression level of isozyme SCD-1 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | palmitoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCD-1 | - |
Homo sapiens |
stearoyl-CoA desaturase | - |
Homo sapiens |
stearoyl-CoA desaturase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | stearoyl-CoA desaturase 1 (SCD-1) inhibition blocks hepatitis C virus replication and formation of hepatitis C virus-induced specialized membranes. Inhibition of stearoyl-CoA desaturase 1 halts the biosynthesis of unsaturated fatty acids, such as oleic acid, and negatively modulates HCV replication. Unsaturated fatty acids play key roles in membrane curvature and fluidity, SCD-1 inhibition disrupts the integrity of membranous HCV replication complexes and renders HCV RNA susceptible to nuclease-mediated degradation | Homo sapiens |
physiological function | stearoyl-CoA desaturase is a key enzyme in the biosynthesis of oleic acid, role of SCD-1 in the fatty acid biosynthesis pathway, schematic overview. SCD-1 catalyzes the fatty acids desaturation at carbon-9 forming a cis-double bond. The SCD-1 produced products, palmitoleoyl-CoA (C16:1) and oleoyl-CoA (C18:1), are directly incorporated in triglycerides, cholesterol esters, and phospholipids. These lipids are crucial to the formation of cytosolic lipid droplets, luminal lipid domains, and lipid-rich membranes, which are platforms for hepatitis C virus replication and assembly. Analysis of the influence of SCD-1 on the nuclease and detergent resistant properties of viral RNA at replication complexes | Homo sapiens |