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Literature summary for 1.14.19.1 extracted from
- Thermal thresholds of lipid restructuring and DELTA9-desaturase expression in the liver of carp (Cyprinus carpio L.) (2000), J. Exp. Biol., 203, 641-650.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 reductase | - |
Rattus norvegicus | |
| cytochrome b5 reductase | NADH-dependent | Cyprinus carpio |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| endoplasmic reticulum | - |
Cyprinus carpio | 5783 | - |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Cyprinus carpio | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
- |
Cyprinus carpio |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyprinus carpio | - |
L. | - |
| Cyprinus carpio L. | - |
L. | - |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyprinus carpio |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | presence of two desaturase genes, isomers CDS1 and CDS2. Cooling treatment of carp causes significant changes to the activity of the DELTA9-desaturase. Modest cooling of carp shows an increase in acticity without any increase in the amount of desaturase protein, suggesting the activation of the pre-existing inactive latent enzyme, possibly by a post-translational mechanism | Cyprinus carpio |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| liver | - |
Cyprinus carpio | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, saline solution, 150 mM NaCl, 0.1 mM EDTA, 20 mM HEPES, pH 7.4 | Cyprinus carpio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + AH2 + O2 | - |
Cyprinus carpio | palmitoleoyl-CoA + A | - |
? |  |
| palmitoyl-CoA + AH2 + O2 | - |
Cyprinus carpio L. | palmitoleoyl-CoA + A | - |
? | |
| stearoyl-CoA + AH2 + O2 | - |
Cyprinus carpio | oleoyl-CoA + A + H2O | - |
? | |
| stearoyl-CoA + AH2 + O2 | - |
Cyprinus carpio L. | oleoyl-CoA + A + H2O | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | - |
Rattus norvegicus | |
| cytochrome b5 | - |
Cyprinus carpio | |
| NADH | - |
Rattus norvegicus | |
| NADH | - |
Cyprinus carpio | |
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