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Literature summary for 1.14.19.1 extracted from
- Purification and properties of hen liver microsomal terminal enzyme involved in stearoyl coenzyme A desaturation and its quantitation in neonatal chicks (1979), J. Biol. Chem., 254, 6362-6369.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 reductase | NADH-specific, required, EC 1.6.2.2 | Gallus gallus | |
| cytochrome b5 reductase | NADH-specific, required, EC 1.6.2.2 | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Gallus gallus |  |
| azide | - |
Rattus norvegicus | |
| CN- | - |
Gallus gallus | |
| CN- | - |
Rattus norvegicus | |
| decanoyl-CoA | competitive inhibition of enzyme activity greater than 95% at 0.002 mM | Gallus gallus | |
| dodecanoyl-CoA | competitive inhibition of enzyme activity greater than 90% at 0.002 mM | Gallus gallus | |
| additional information | N-ethylmaleimide and iodoacetamide have no inhibitory effect | Gallus gallus | |
| additional information | - |
Rattus norvegicus | |
| oleoyl-CoA | not competitively | Gallus gallus | |
| p-chloromercuribenzoate | prevention by stearoyl-CoA; reversed by beta-mercaptoethanol | Gallus gallus | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
| p-hydroxymercuribenzoate | - |
Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| endoplasmic reticulum | cytoplasmic surface of | Gallus gallus | 5783 | - |
| microsome | membrane bound | Gallus gallus | - |
- |
| microsome | membrane bound | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | contains a non-heme iron | Gallus gallus | |
| Fe2+ | contains a non-heme iron | Rattus norvegicus | |
| additional information | enzyme contains active sulfhydryl groups | Gallus gallus | |
| additional information | enzyme contains active sulfhydryl groups | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33600 | - |
x * 33600, SDS-PAGE | Gallus gallus |
| 55000 | - |
x * 55000 | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anionic and nonionic detergents, solubilization and ion-exchange | Gallus gallus |
| ion-exchange | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | introduction of double bond at position 9 | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -60°C, 0.12 M sodium phosphate buffer, pH 8.0, 0.5% Triton X-100, stable for several months | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-CoA + NADH + O2 | 59% activity of stearoyl-CoA | Gallus gallus | ? | - |
? | |
| palmitoyl-CoA + NADH + O2 | - |
Rattus norvegicus | palmitoleoyl-CoA + NAD+ + H2O | - |
? | |
| palmitoyl-CoA + NADH + O2 | 62% activity of stearoyl-CoA | Gallus gallus | palmitoleoyl-CoA + NAD+ + H2O | - |
? | |
| stearoyl-CoA + NADH + O2 | - |
Gallus gallus | oleoyl-CoA + NAD+ + H2O | - |
? | |
| stearoyl-CoA + NADH + O2 | - |
Rattus norvegicus | oleoyl-CoA + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000 | Rattus norvegicus |
| ? | x * 33600, SDS-PAGE | Gallus gallus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
50% loss of activity after repeated freezing and thawing | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Gallus gallus |
| 7.2 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | - |
Gallus gallus | |
| cytochrome b5 | required | Rattus norvegicus | |
| NADH | - |
Gallus gallus | |
| NADH | - |
Rattus norvegicus | |
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