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Literature summary for 1.14.18.9 extracted from

  • Nose, H.; Miyara, T.; Kushida, N.; Hoshiko, S.
    Isolation of temperature-sensitive Saccharomyces cerevisiae with a mutation in erg25 for C-4 sterol methyl oxidase (2002), J. Antibiot., 55, 962-968.
    View publication on PubMed
Search for more literature for 1.14.18.9

Application

Application Comment Organism
agriculture enzyme is an antifungal target Saccharomyces cerevisiae
medicine enzyme is an antifungal target Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of wild-type and mutant strains Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of a disruption mutant dERG25 Saccharomyces cerevisiae
N48D/V133A/F135S construction of diverse mutants by random mutagenesis, isolation of the temperature-sensitive mutant strain mERG25 which grows normally at 28°C, but fails to grow at 38°C showing an altered sterol profile Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-heme iron Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 Saccharomyces cerevisiae step 3 of 3 of the overall reaction 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
 ?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 Saccharomyces cerevisiae step 1 of 3 of the overall reaction 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
 ?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 Saccharomyces cerevisiae step 2 of 3 of the overall reaction 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
 ?
additional information Saccharomyces cerevisiae enzyme is a key enzyme in ergosterol biosynthetic pathway in fungi, determination and analysis of sterol content in wild-type and mutant strains ?
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 step 3 of 3 of the overall reaction Saccharomyces cerevisiae 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
 ?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 step 1 of 3 of the overall reaction Saccharomyces cerevisiae 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
 ?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 step 2 of 3 of the overall reaction Saccharomyces cerevisiae 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
 ?
additional information enzyme is a key enzyme in ergosterol biosynthetic pathway in fungi, determination and analysis of sterol content in wild-type and mutant strains Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme possesses 3 His-rich motifs common for non-heme diiron-containing enzymes Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
C-4 sterol methyl oxidase
-
 Saccharomyces cerevisiae
ERG25p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Saccharomyces cerevisiae