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Literature summary for 1.14.18.9 extracted from
- Investigation of the component reactions of oxidative sterol demethylation. Evidence against participation of cytochrome P-450 (1968), J. Biol. Chem., 243, 4966-4972.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | - |
Rattus norvegicus |  |
| cytochrome c | - |
Rattus norvegicus | |
| Trypsin | 30 min treatment, 18% of original concentration of cytochrome b5 left | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley | - |
| Rattus norvegicus | - |
male, adult | - |
| Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, ammonium sulfate fractionation, affinity chromatography | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O | aerobic hydroxylation | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme does not involve P-450 | Rattus norvegicus |
| 0.000201 | - |
recovered after Lubrol treatment, 1% of original cytochrome b5 concentration in microsomes, 4-fold that observed for microsomes, maybe cytochrome b5 does not participate in hydroxylation | Rattus norvegicus |
| 0.00356 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| microsomes frozen, buffers contain 1 mM glutathione, no loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | - |
Rattus norvegicus | 4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + CO2 | - |
? | |
| 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | - |
Rattus norvegicus Sprague-Dawley | 4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + CO2 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 10 to 15% more active with NADH than with NADPH | Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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