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Literature summary for 1.14.18.5 extracted from

  • Idkowiak-Baldys, J.; Takemoto, J.Y.; Grilley, M.M.
    Structure-function studies of yeast C-4 sphingolipid long chain base hydroxylase (2003), Biochim. Biophys. Acta, 1618, 17-24.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D271T 98% of wild-type activity Saccharomyces cerevisiae
F174L complete loss of activity Saccharomyces cerevisiae
H176A complete loss of activity Saccharomyces cerevisiae
H180A complete loss of activity Saccharomyces cerevisiae
H190A complete loss of activity Saccharomyces cerevisiae
H193A complete loss of activity Saccharomyces cerevisiae
H194A complete loss of activity Saccharomyces cerevisiae
H249A complete loss of activity Saccharomyces cerevisiae
H270A complete loss of activity Saccharomyces cerevisiae
H273A complete loss of activity Saccharomyces cerevisiae
H274A complete loss of activity Saccharomyces cerevisiae
L196W complete loss of activity Saccharomyces cerevisiae
additional information the replacement of any one of conserved His residues of three histidine-rich motifs with an alanine eliminates hydroxylase activity in vivo and in vitro. Residues Phe 174, Asn 182, Ser 191, Leu 196, Pro 199, Asn 266, Tyr 269, Asp 271 and Gln 275 appear to be additionally important elements of the active site but their conversion into corresponding yeast DELTA7-sterol-C5(6)-desaturase Erg3p residues does not lead to a gain in desaturase activity Saccharomyces cerevisiae
N182P complete loss of activity Saccharomyces cerevisiae
N266G complete loss of activity Saccharomyces cerevisiae
P199C complete loss of activity Saccharomyces cerevisiae
Q275L complete loss of activity Saccharomyces cerevisiae
S191K 99% of wild-type activity Saccharomyces cerevisiae
Y269C complete loss of activity Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P38992
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrosphingosine + NADPH + H+ + O2
-
Saccharomyces cerevisiae phytosphingosine + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
sphingolipid C4-hydroxylase
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Saccharomyces cerevisiae
SUR2
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Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function C-4 hydroxylation of sphingolipids is important for the antifungal action of syringomycin E. Mutants with defects in C-4 hydroxylated sphingoid base biosynthesis are resistant to this antifungal agent Saccharomyces cerevisiae