BRENDA - Enzyme Database show
show all sequences of 1.14.18.4

Secretory production of ricinoleic acid in fission yeast Schizosaccharomyces pombe

Yazawa, H.; Kumagai, H.; Uemura, H.; Appl. Microbiol. Biotechnol. 97, 8663-8671 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
overexpression of multicopy suppressor Plg7, encoding phospholipase A2, in combinantion with oleate DELTA12-hydroxylase gene FAH12 enables Schizosaccharomyces pombe cells to secrete free ricinoleic acid into culture media. The FAH12 integrant in the absence of the overexpressed plg7 reaches 200 microg/ml of intracellular ricinoleic acid and only 69.3 microg/ml in culture media. The FAH12 integrant harboring the plg7 multicopy plasmid secretes ricinoleic acid in the media (184.5 microg/ml) without decreasing the amount in the cells
Claviceps purpurea
Cloned(Commentary)
Commentary
Organism
expression in Schizosaccharomyces pombe
Claviceps purpurea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Claviceps purpurea
-
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
overexpression of multicopy suppressor Plg7, encoding phospholipase A2, in combinantion with oleate DELTA12-hydroxylase gene FAH12 enables Schizosaccharomyces pombe cells to secrete free ricinoleic acid into culture media. The FAH12 integrant in the absence of the overexpressed plg7 reaches 200 microg/ml of intracellular ricinoleic acid and only 69.3 microg/ml in culture media. The FAH12 integrant harboring the plg7 multicopy plasmid secretes ricinoleic acid in the media (184.5 microg/ml) without decreasing the amount in the cells
Claviceps purpurea
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Schizosaccharomyces pombe
Claviceps purpurea
Other publictions for EC 1.14.18.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746157
Venegas-Caleron
Molecular and biochemical cha ...
Ricinus communis, Ricinus communis OLE-1
Planta
244
245-258
2016
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7
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734438
Meesapyodsuk
Metabolic engineering of Pichi ...
Claviceps purpurea
J. Lipid Res.
56
2102-2109
2015
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744145
Yazawa
Suppression of ricinoleic aci ...
Claviceps purpurea
Appl. Microbiol. Biotechnol.
98
9325-9337
2014
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733188
Yazawa
Secretory production of ricino ...
Claviceps purpurea
Appl. Microbiol. Biotechnol.
97
8663-8671
2013
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733184
Holic
Engineered high content of ric ...
Claviceps purpurea
Appl. Microbiol. Biotechnol.
95
179-187
2012
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697014
Dauk
-
The role of diacylglycerol acy ...
Ricinus communis
Botany
87
552-560
2009
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689616
Meesapyodsuk
An oleate hydroxylase from the ...
Claviceps purpurea
Plant Physiol.
147
1325-1333
2008
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1
1
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689659
Dauk
A FAD2 homologue from Lesquere ...
Physaria lindheimeri
Plant Sci.
173
43-49
2007
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660300
Smith
Heterologous expression of a f ...
Physaria fendleri, Ricinus communis
Planta
217
507-516
2003
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734603
Lin
Molecular species of PC and PE ...
Ricinus communis
Lipids
37
991-995
2002
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438917
Broun
A bifunctional oleate 12-hydro ...
Physaria fendleri
Plant J.
13
201-210
1998
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438918
Lin
Characterization of oleoyl-12- ...
Ricinus communis
Lipids
31
571-577
1996
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438916
Van de Loo
An oleate 12-hydroxylase from ...
Ricinus communis
Proc. Natl. Acad. Sci. USA
92
6743-6747
1995
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438919
Richards
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Localization and possible subs ...
Ricinus communis
Plant Physiol. Biochem.
31
89-94
1993
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438915
Moreau
Recent studies of the enzymic ...
Ricinus communis
Plant Physiol.
67
672-676
1981
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