Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.18.3 extracted from

  • Culpepper, M.A.; Cutsail, G.E.; Gunderson, W.A.; Hoffman, B.M.; Rosenzweig, A.C.
    Identification of the valence and coordination environment of the particulate methane monooxygenase copper centers by advanced EPR characterization (2014), J. Am. Chem. Soc., 136, 11767-11775 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.18.3

Protein Variants

Protein Variants Comment Organism
H33A/H137A/H137A/H139A/H48N site-directed mutagenesis Methylococcus capsulatus
H33A/H137A/H139A site-directed mutagenesis Methylococcus capsulatus
H48N site-directed mutagenesis Methylococcus capsulatus
H72A site-directed mutagenesis Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methylococcus capsulatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the dicopper site is located at the N-terminus of the pmoB subunit, and conserved residues His33, His137, and His139 coordinate the copper ions. Copper center modeling: the first site is modeled as a single copper ion coordinated by residues His48 and His72 and is not present in other pMMOs. The second site, located near the membrane interface, is coordinated by residues His33, His137, and His139 and is highly conserved among pMMOs and related enzymes. The EPR measurements indicate that the dicopper site in pMMO contains one Cu(I) ion and one Cu(II) ion, proposed as a valence-localized mixed-valence Cu(I)Cu(II) pair, and that the monocopper site is present as Cu(I). The 1H ENDOR measurements show that the Cu(II) is not coordinated by a HxO ligand, so the two ions of the Cu(I)Cu(II) pair cannot be bridged by a hydroxo group in the as-isolated samples. The measurements do not rule out an oxo bridge Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus G1UBD1 AND Q607G3 alpha- and beta-subunits
-
Methylococcus capsulatus Bath G1UBD1 AND Q607G3 alpha- and beta-subunits
-

Subunits

Subunits Comment Organism
heterotrimer alpha3beta3gamma3 trimer comprising three copies each of the pmoB (alpha), pmoA (beta), and pmoC (gamma) subunits Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
particulate methane monooxygenase
-
 Methylococcus capsulatus
pMMO
-
 Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Methylococcus capsulatus