Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bacteriohemerythrin | enhances enzyme activity. The maximum activity is observed at a enzyme to bacteriohemerythrin concentration ratio of 4:1 | Methylococcus capsulatus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
duroquinone | noncompetitive inhibitor | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Methylococcus capsulatus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | the enzyme contains 13 copper ions | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
27000 | - |
1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
45000 | - |
1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MonoQ 10/100 GL column chromatography and Sephacryl S100 gel filtration | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1037 | - |
at 45°C and pH 7.0, with NADH as cosubstrate, in the presence of bacteriohemerythrin | Methylococcus capsulatus |
0.1228 | - |
at 45°C and pH 7.0, with duroquinol as cosubstrate, in the presence of bacteriohemerythrin | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propylene + duroquinol + O2 | - |
Methylococcus capsulatus | propylene oxide + duroquinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
particulate methane monooxygenase | - |
Methylococcus capsulatus |
pMMO | - |
Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |