Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the active center of pMH is located in the beta-subunit | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetylene | is a competitive inhibitor of pMH, interacts with its substrate-binding center | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Methylococcus capsulatus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | pMH contains 2-4 atoms of copper per a minimum molecular weight of 99 kDa | Methylococcus capsulatus | |
Iron | pMH contains 1 or 2 atoms of nonheme iron | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE | Methylococcus capsulatus |
27000 | - |
pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE | Methylococcus capsulatus |
47000 | - |
pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE | Methylococcus capsulatus |
99000 | - |
- |
Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Methylococcus capsulatus M | - |
- |
- |
Purification (Comment) | Organism |
---|---|
particulate form of methane hydroxylase (pMH) obtained by ion exchange and hydrophobic chromatography | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus | methanol + acceptor + H2O | - |
? | |
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus M | methanol + acceptor + H2O | - |
? | |
Mn2+ + H2O2 | - |
Methylococcus capsulatus | ? | - |
? | |
Mn2+ + H2O2 | - |
Methylococcus capsulatus M | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
membrane-bound methane monooxygenase | - |
Methylococcus capsulatus |
methane hydroxylase | - |
Methylococcus capsulatus |
PMH | - |
Methylococcus capsulatus |
pMMO | - |
Methylococcus capsulatus |