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Literature summary for 1.14.18.3 extracted from

  • Vasilev, V.I.; Tikhonova, T.V.; Gvozdev, R.I.; Tukhvatullin, I.A.; Popov, V.O.
    Optimization of solubilization and purification procedures for the hydroxylase component of membrane-bound methane monooxygenase from Methylococcus capsulatus strain M (2006), Biochemistry (Moscow), 71, 1329-1335.
    View publication on PubMed
Search for more literature for 1.14.18.3

Inhibitors

Inhibitors Comment Organism Structure
duroquinol increasing duroquinol concentration above 70 mM causes almost total inhibition of enzyme activity Methylococcus capsulatus
NaCl decrease in activity might be due to reduced enzyme solubility with increasing NaCl concentrations Methylococcus capsulatus
propylene oxide product inhibition at higher concentration Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Methylococcus capsulatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme contains three Co2+ ions per enzyme molecule Methylococcus capsulatus
Fe2+ the enzyme contains one Fe2+ ion per enzyme molecule Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus
26000
-
 x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus
47000
-
 x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus
660000
-
 solubilized enzyme, gel filtration Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + reduced acceptor + H* + O2 Methylococcus capsulatus
-
 methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2 Methylococcus capsulatus M
-
 methanol + acceptor + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus M
-
-
-

Purification (Commentary)

Purification (Comment) Organism
optimization of solubilization and purification procedure for the hydroxylase component of membrane-bound enzyme, purification to homogeneity involves solubilization by dodexylbeta-D-maltoside, ion exchange chromatography and gel filtration, the purification includes the loss of the reductase component Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 optimization of a coupled assay method with propylene as substrate and a duroquinol-based NADH regeneration system Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus M methanol + acceptor + H2O
-
 ?
Mn2+ + H2O2
-
 Methylococcus capsulatus ?
-
 ?
Mn2+ + H2O2
-
 Methylococcus capsulatus M ?
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
MMO
-
 Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.3 assay at Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Methylococcus capsulatus