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Literature summary for 1.14.18.2 extracted from

  • Gao, H.; Zhao, C.; Xiang, X.; Li, Y.; Zhao, Y.; Li, Z.; Pan, D.; Dai, Y.; Hara, H.; Cooper, D.K.; Cai, Z.; Mou, L.
    Production of alpha1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning (2017), J. Reprod. Dev., 63, 17-26 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
generation of bi-allelic GGTA1 and CMAH double-knockout cells Sus scrofa

Protein Variants

Protein Variants Comment Organism
additional information production of alpha1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and cloning in porcine fetal fibroblast cells. Generation of bi-allelic GGTA1 and CMAH double-knockout cells, GGTA1/CMAH double-knockout (DKO) cells are cloned to produce piglets. Compared to cells from GGTA1-knockout pigs, human antibody binding and antibody-mediated complement-dependent cytotoxicity are significantly reduced in cells from GGTA1/CMAH DKO pigs, demonstrating that the pigs might exhibit reduced humoral rejection in xenotransplantation Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Sus scrofa 9986
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ Sus scrofa
-
 CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 Chinese domestic miniature Wuzhishan pigs
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Sus scrofa CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CMAH
-
 Sus scrofa
cytidine monophosphate-N-acetylneuraminic acid hydroxylase
-
 Sus scrofa

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
 Sus scrofa

General Information

General Information Comment Organism
evolution detailed phylogenetic analysis, overview Sus scrofa
physiological function enzyme CMP-N-acetylneuraminic acid hydroxylase (CMAH) is responsible for the synthesis of N-glycolylneuraminic acid (Neu5Gc), a sialic acid present on the cell surface proteins of most deuterostomes. CMAH is the only enzyme capable of synthetizing Neu5Gc Sus scrofa