Application | Comment | Organism |
---|---|---|
synthesis | enzyme CMAH can be used for large-scale production of N-glycolylneuraminic acid | Chlorocebus aethiops |
Cloned (Comment) | Organism |
---|---|
functional recombinant expression of secretory His-tagged enzyme, containing an N-terminal secretion signal peptide, in Spodoptera frugiperda Sf9 cells using the baculovirus infection method, large scale enzyme production | Chlorocebus aethiops |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ | Chlorocebus aethiops | - |
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorocebus aethiops | - |
- |
- |
no activity in Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the secreted recombinant His-tagged enzyme contains N-glycans | Chlorocebus aethiops |
Purification (Comment) | Organism |
---|---|
recombinant secretory His-tagged enzyme from Spodoptera frugiperda Sf9 cell culture supernatant by affinity chromatography and ultrafiltration | Chlorocebus aethiops |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
COS-7 cell | - |
Chlorocebus aethiops | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Chlorocebus aethiops | CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x + 65000, secreted recombinant His-tagged enzyme, SDS-PAGE | Chlorocebus aethiops |
Synonyms | Comment | Organism |
---|---|---|
CMAH | - |
Chlorocebus aethiops |
CMP Neu5Ac hydroxylase | - |
Chlorocebus aethiops |
cytidine monophosphate-N-acetylneuraminic acid hydroxylase | - |
Chlorocebus aethiops |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Chlorocebus aethiops |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Chlorocebus aethiops |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Chlorocebus aethiops |
General Information | Comment | Organism |
---|---|---|
additional information | sequence-based, three-dimensional enzyme structure modeling, overview | Chlorocebus aethiops |
physiological function | the enzyme is essential for N-glycolylneuraminic acid (Neu5Gc) synthesis | Chlorocebus aethiops |