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Literature summary for 1.14.18.11 extracted from

  • Pascal, S.; Taton, M.; Rahier, A.
    Plant sterol biosynthesis. Identification and characterization of two distinct microsomal oxidative enzymatic systems involved in sterol C4-demethylation (1993), J. Biol. Chem., 268, 11639-11654 .
    View publication on PubMed
Search for more literature for 1.14.18.11

Inhibitors

Inhibitors Comment Organism Structure
cyanide
-
 Zea mays
cytochrome c
-
 Zea mays
menadione 34% residual activity at 0.05 mM Zea mays
additional information not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis Zea mays
orthophenanthroline 47% residual activity at 1 mM Zea mays
stigmastan-3beta,5alpha,6alpha-triol effectively inhibited by stigmastan-3beta,5alpha,6alpha-triol Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Zea mays
-
-

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
 Zea mays (3beta,4alpha,5alpha,24Z)-3-hydroxystigmasta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
 ir
24-ethylidenelophenol + NAD(P)H + O2 + H+ 100% activity Zea mays ?
-
 ?
24-methylidenelophenol + NAD(P)H + H+ 59% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
24zeta(241)-dihydrocycloeucalenol + NAD(P)H + H+ 10% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
cycloeucalenol + NAD(P)H + H+ 10% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
lophenol + NAD(P)H + H+ 38% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
additional information no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol Zea mays ?
-
 ?

Synonyms

Synonyms Comment Organism
4alpha-methyl oxidase
-
 Zea mays
4alpha-methylsterol C4 oxidase
-
 Zea mays

Cofactor

Cofactor Comment Organism Structure
NADH required Zea mays
NADPH required Zea mays

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.035
-
 using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication Zea mays cytochrome c
0.05
-
 using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication Zea mays cyanide