Cloned (Comment) | Organism |
---|---|
the wild-type sequence coding for the enzyme is obtained by restriction digestion of the entire cDNA sequence, subcloning in Escherichia coli H10Bac, and recombinant expression of His-tagged enzyme in Spdoptera frugiperda Sf9 cells via the baculovirus transfection method | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
L-tyrosine | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
0.34 | - |
L-Dopa | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
0.6 | - |
4-tert-butylcatechol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
0.65 | - |
4-tert-butylcatechol | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
1.25 | - |
3-(3,5-dihydroxyphenyl)-1-propanoic acid | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
1.5 | - |
L-Dopa | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
1.9 | - |
L-dopa methyl ester | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
2.5 | - |
4-methylcatechol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
4.2 | - |
L-dopa methyl ester | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
4.5 | - |
catechol | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
5.1 | - |
L-tyrosine | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
11 | - |
3,4-dihydroxyphenylacetic acid | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
16 | - |
dopamine | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
19 | - |
3,4-dihydroxyphenylacetic acid | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
32 | - |
catechol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
50 | - |
dopamine | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
58 | - |
L-isoproterenol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
230 | - |
L-isoproterenol | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a transmembrane enzyme | Homo sapiens | 16020 | - |
additional information | the enzyme sequence contains a melanosome-directing signal sequence | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper-containing enzyme, two Cu2+ ions, CuA and CuB | Homo sapiens | |
Cu2+ | a copper-containing enzyme, two Cu2+ ions, CuA and CuB | Streptomyces antibioticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | Homo sapiens | - |
2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | Streptomyces antibioticus | - |
2 dopaquinone + 2 H2O | - |
? | |
L-tyrosine + O2 | Homo sapiens | - |
dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | Streptomyces antibioticus | - |
dopaquinone + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14679 | - |
- |
Streptomyces antibioticus | P07524 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Spdoptera frugiperda Sf9 cells by nickel affinity chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | - |
Homo sapiens | 2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | - |
Streptomyces antibioticus | 2 dopaquinone + 2 H2O | - |
? | |
3,4-dihydroxyphenylacetic acid + O2 | - |
Homo sapiens | ? | - |
? | |
3,4-dihydroxyphenylacetic acid + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
3-(3,5-dihydroxyphenyl)-1-propanoic acid + O2 | low activity | Streptomyces antibioticus | ? | - |
? | |
4-methylcatechol + O2 | - |
Homo sapiens | ? | - |
? | |
4-methylcatechol + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
4-tert-butylcatechol + O2 | - |
Homo sapiens | ? | - |
? | |
4-tert-butylcatechol + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
catechol + O2 | - |
Homo sapiens | ? | - |
? | |
catechol + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
dopamine + O2 | - |
Homo sapiens | ? | - |
? | |
dopamine + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
L-dopa methyl ester + O2 | - |
Homo sapiens | ? | - |
? | |
L-dopa methyl ester + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
L-isoproterenol + O2 | - |
Homo sapiens | ? | - |
? | |
L-isoproterenol + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
L-tyrosine + O2 | - |
Homo sapiens | dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | - |
Streptomyces antibioticus | dopaquinone + H2O | - |
? | |
additional information | no activity with [3-(3,5-dihydroxyphenyl)-1-propanoic acid] | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000 + x * 57000, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Streptomyces antibioticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.28 | - |
L-isoproterenol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
2.6 | - |
L-tyrosine | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
3 | - |
3-(3,5-dihydroxyphenyl)-1-propanoic acid | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
5.8 | - |
L-isoproterenol | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
5.9 | - |
3,4-dihydroxyphenylacetic acid | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
7.9 | - |
L-tyrosine | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
12 | - |
4-methylcatechol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
20.5 | - |
4-tert-butylcatechol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
38.1 | - |
L-Dopa | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
44 | - |
catechol | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
57 | - |
L-dopa methyl ester | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
61 | - |
dopamine | recombinant enzyme, pH 7.2, 37°C | Homo sapiens | |
80 | - |
catechol | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
113 | - |
3,4-dihydroxyphenylacetic acid | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
187 | - |
L-dopa methyl ester | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
197 | - |
L-Dopa | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
210 | - |
dopamine | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus | |
1650 | - |
4-tert-butylcatechol | recombinant enzyme, pH 7.2, 37°C | Streptomyces antibioticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
7.2 | - |
assay at | Streptomyces antibioticus |
General Information | Comment | Organism |
---|---|---|
physiological function | human tyrosinase is the first enzyme of the multistep process of melanogenesis. It catalyzes the hydroxylation of L-tyrosine to L-dihydroxyphenylalanine and the following oxidation of o-diphenol to the corresponding quinone, L-dopaquinone | Homo sapiens |
physiological function | tyrosinase is the first enzyme of the multistep process of melanogenesis. It catalyzes the hydroxylation of L-tyrosine to L-dihydroxyphenylalanine and the following oxidation of o-diphenol to the corresponding quinone, L-dopaquinone | Streptomyces antibioticus |