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Literature summary for 1.14.18.1 extracted from

  • Teng, J.; Gong, Z.; Deng, Y.; Chen, L.; Li, Q.; Shao, Y.; Lin, L.; Xiao, W.
    Purification, characterization and enzymatic synthesis of theaflavins of polyphenol oxidase isozymes from tea leaf (Camellia sinensis) (2017), LWT-Food Sci. Technol., 84, 263-270 .
No PubMed abstract available
Search for more literature for 1.14.18.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 catechol pH 5.6, 37°C Camellia sinensis
5.8
-
 pyrogallate pH 5.6, 37°C Camellia sinensis
6.9
-
 epigallocatechin gallate pH 5.6, 37°C Camellia sinensis
8
-
 pyrogallate pH 5.6, 37°C Camellia sinensis
16
-
 catechol pH 5.6, 37°C Camellia sinensis
21
-
 epigallocatechin pH 5.6, 37°C Camellia sinensis
28
-
 epigallocatechin pH 5.6, 37°C Camellia sinensis
32
-
 epigallocatechin gallate pH 5.6, 37°C Camellia sinensis
38
-
 guaiacol pH 5.6, 37°C Camellia sinensis
90
-
 L-tyrosine pH 5.6, 37°C Camellia sinensis
145
-
 L-tyrosine pH 5.6, 37°C Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 catechol + O2 Camellia sinensis
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
epigallocatechin + O2 Camellia sinensis
-
 ? + 2 H2O
-
 ?
epigallocatechin gallate + O2 Camellia sinensis
-
 ? + 2 H2O
-
 ?
guaiacol + O2 Camellia sinensis
-
 ?
-
 ?
L-tyrosine + O2 Camellia sinensis
-
 dopaquinone + H2O
-
 ?
additional information Camellia sinensis synthesis of theaflavins of polyphenol oxidase isozymes from tea leaves ?
-
 ?
pyrogallate + O2 Camellia sinensis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis C0L3S4
-
-
Camellia sinensis Q68NI4
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from leaves by ultrafiltration, gel filtration, and twofold anion exchange chromatography Camellia sinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
 Camellia sinensis 2 1,2-benzoquinone + 2 H2O
-
 ?
epigallocatechin + O2
-
 Camellia sinensis ? + 2 H2O
-
 ?
epigallocatechin gallate + O2
-
 Camellia sinensis ? + 2 H2O
-
 ?
guaiacol + O2
-
 Camellia sinensis ?
-
 ?
L-tyrosine + O2
-
 Camellia sinensis dopaquinone + H2O
-
 ?
additional information synthesis of theaflavins of polyphenol oxidase isozymes from tea leaves Camellia sinensis ?
-
 ?
additional information no activity with guaiacol. Synthesis of theaflavin an theaflavin gallates from different substrate by polyphenol oxidase, overview Camellia sinensis ?
-
 ?
additional information synthesis of theaflavin an theaflavin gallates from different substrate by polyphenol oxidase, overview Camellia sinensis ?
-
 ?
pyrogallate + O2
-
 Camellia sinensis ?
-
 ?
pyrogallol + O2
-
 Camellia sinensis ?
-
 ?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Camellia sinensis
PPO1
-
 Camellia sinensis
PPO2
-
 Camellia sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Camellia sinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 purified isozyme PPO1, 5 min, 40% activity remaining Camellia sinensis
70
-
 purified isozyme PPO1, 5 min, 75% activity remaining Camellia sinensis
80
-
 purified isozyme PPO1, 5 min, 15% activity remaining Camellia sinensis
80
-
 purified isozyme PPO1, 5 min, inactivation Camellia sinensis
90
-
 purified isozyme PPO1, 3 min, inactivation Camellia sinensis
90
-
 purified isozyme PPO1, 5 min, inactivation Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 assay at Camellia sinensis