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Literature summary for 1.14.18.1 extracted from

  • Ilesanmi, O.; Ojopagogo, Y.; Adewale, I.
    Kinetic characteristics of purified tyrosinase from different species of Dioscorea (YAM) in aqueous and non-aqueous systems (2014), J. Mol. Catal. B, 108, 111-117 .
No PubMed abstract available
Search for more literature for 1.14.18.1

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzymes is activated in the presence of 1-40% water-immiscible ether Dioscorea cayenensis subsp. rotundata
additional information the enzymes is activated in the presence of 1-40% water-immiscible ether Colocasia esculenta
additional information the enzymes is activated in the presence of 1-40% water-immiscible ether Dioscorea praehensilis
additional information the enzymes is activated in the presence of 1-40% water-immiscible ether Dioscorea alata

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Colocasia esculenta
2-mercaptoethanol
-
 Dioscorea alata
2-mercaptoethanol
-
 Dioscorea cayenensis subsp. rotundata
2-mercaptoethanol
-
 Dioscorea praehensilis
EDTA
-
 Colocasia esculenta
EDTA
-
 Dioscorea alata
EDTA
-
 Dioscorea cayenensis subsp. rotundata
EDTA
-
 Dioscorea praehensilis
glutathione
-
 Colocasia esculenta
glutathione
-
 Dioscorea alata
glutathione
-
 Dioscorea cayenensis subsp. rotundata
glutathione
-
 Dioscorea praehensilis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper-containing enzyme Dioscorea cayenensis subsp. rotundata
Cu2+ a copper-containing enzyme Colocasia esculenta
Cu2+ a copper-containing enzyme Dioscorea praehensilis
Cu2+ a copper-containing enzyme Dioscorea alata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41300
-
 gel filtration Colocasia esculenta
53400
-
 gel filtration Dioscorea cayenensis subsp. rotundata
54800
-
 gel filtration Dioscorea alata
57500
-
 gel filtration Dioscorea praehensilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 catechol + O2 Dioscorea cayenensis subsp. rotundata
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 Colocasia esculenta
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 Dioscorea praehensilis
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 Dioscorea alata
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
2 L-dopa + O2 Dioscorea cayenensis subsp. rotundata
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Colocasia esculenta
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Dioscorea praehensilis
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Dioscorea alata
-
 2 dopaquinone + 2 H2O
-
 ?
caffeic acid + O2 Dioscorea cayenensis subsp. rotundata
-
 ?
-
 ?
caffeic acid + O2 Colocasia esculenta
-
 ?
-
 ?
caffeic acid + O2 Dioscorea praehensilis
-
 ?
-
 ?
caffeic acid + O2 Dioscorea alata
-
 ?
-
 ?
DL-tyrosine + O2 Dioscorea cayenensis subsp. rotundata
-
 dopaquinone + H2O
-
 ?
DL-tyrosine + O2 Colocasia esculenta
-
 dopaquinone + H2O
-
 ?
DL-tyrosine + O2 Dioscorea praehensilis
-
 dopaquinone + H2O
-
 ?
DL-tyrosine + O2 Dioscorea alata
-
 dopaquinone + H2O
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 50% activity remains in 40% ethanol, protein precipitation at 70% Colocasia esculenta
Ethanol below 20% activity remains in 40% ethanol Dioscorea cayenensis subsp. rotundata
Ethanol below 20% activity remains in 40% ethanol Dioscorea alata
Ethanol below 20% activity remains in 40% ethanol, protein precipitation at 90% Dioscorea praehensilis
ether the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h Dioscorea cayenensis subsp. rotundata
ether the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h Colocasia esculenta
ether the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h Dioscorea praehensilis
ether the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h Dioscorea alata

Organism

Organism UniProt Comment Textmining
Colocasia esculenta
-
 cultivars are grown around Ile-Ife, South Western Nigeria
-
Dioscorea alata
-
 cultivars are grown around Ile-Ife, South Western Nigeria
-
Dioscorea cayenensis subsp. rotundata
-
 cultivars are grown around Ile-Ife, South Western Nigeria
-
Dioscorea praehensilis
-
 cultivars are grown around Ile-Ife, South Western Nigeria
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 11.4fold by cation exchange chromatography, anion exchange chromatography, and gel filtration Dioscorea cayenensis subsp. rotundata
native enzyme 13.0fold by cation exchange chromatography, anion exchange chromatography, and gel filtration Dioscorea alata
native enzyme 4.5fold by cation exchange chromatography, anion exchange chromatography, and gel filtration Dioscorea praehensilis
native enzyme 7.2fold by cation exchange chromatography, anion exchange chromatography, and gel filtration Colocasia esculenta

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Dioscorea cayenensis subsp. rotundata
-
tuber
-
 Colocasia esculenta
-
tuber
-
 Dioscorea alata
-
tuber high enzyme content Dioscorea praehensilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
530
-
 purified enzyme, pH 6.5, 30°C Colocasia esculenta
746
-
 purified enzyme, pH 6.5, 30°C Dioscorea cayenensis subsp. rotundata
1407
-
 purified enzyme, pH 6.5, 30°C Dioscorea alata
1638
-
 purified enzyme, pH 6.5, 30°C Dioscorea praehensilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
 Dioscorea cayenensis subsp. rotundata 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2
-
 Colocasia esculenta 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2
-
 Dioscorea praehensilis 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2
-
 Dioscorea alata 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 51% activity compared to L-dopa Colocasia esculenta 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 85% activity compared to L-dopa Dioscorea alata 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 92% activity compared to L-dopa Dioscorea cayenensis subsp. rotundata 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 92% activity compared to L-dopa Dioscorea praehensilis 2 1,2-benzoquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Dioscorea cayenensis subsp. rotundata 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Colocasia esculenta 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Dioscorea praehensilis 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Dioscorea alata 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 best substrate Dioscorea cayenensis subsp. rotundata 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 best substrate Colocasia esculenta 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 best substrate Dioscorea praehensilis 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 best substrate Dioscorea alata 2 dopaquinone + 2 H2O
-
 ?
caffeic acid + O2
-
 Dioscorea cayenensis subsp. rotundata ?
-
 ?
caffeic acid + O2
-
 Colocasia esculenta ?
-
 ?
caffeic acid + O2
-
 Dioscorea praehensilis ?
-
 ?
caffeic acid + O2
-
 Dioscorea alata ?
-
 ?
caffeic acid + O2 22% activity compared to L-dopa Colocasia esculenta ?
-
 ?
caffeic acid + O2 23% activity compared to L-dopa Dioscorea praehensilis ?
-
 ?
caffeic acid + O2 27% activity compared to L-dopa Dioscorea cayenensis subsp. rotundata ?
-
 ?
caffeic acid + O2 28% activity compared to L-dopa Dioscorea alata ?
-
 ?
DL-tyrosine + O2
-
 Dioscorea cayenensis subsp. rotundata dopaquinone + H2O
-
 ?
DL-tyrosine + O2
-
 Colocasia esculenta dopaquinone + H2O
-
 ?
DL-tyrosine + O2
-
 Dioscorea praehensilis dopaquinone + H2O
-
 ?
DL-tyrosine + O2
-
 Dioscorea alata dopaquinone + H2O
-
 ?
DL-tyrosine + O2 40% activity compared to L-dopa Dioscorea alata dopaquinone + H2O
-
 ?
DL-tyrosine + O2 44% activity compared to L-dopa Colocasia esculenta dopaquinone + H2O
-
 ?
DL-tyrosine + O2 53% activity compared to L-dopa Dioscorea praehensilis dopaquinone + H2O
-
 ?
DL-tyrosine + O2 57% activity compared to L-dopa Dioscorea cayenensis subsp. rotundata dopaquinone + H2O
-
 ?
additional information no activity with resorcinol, phenol, and 2-naphthol Dioscorea cayenensis subsp. rotundata ?
-
 ?
additional information no activity with resorcinol, phenol, and 2-naphthol Colocasia esculenta ?
-
 ?
additional information no activity with resorcinol, phenol, and 2-naphthol Dioscorea praehensilis ?
-
 ?
additional information no activity with resorcinol, phenol, and 2-naphthol Dioscorea alata ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24500, SDS-PAGE Colocasia esculenta
dimer 2 * 25900, SDS-PAGE Dioscorea alata
dimer 2 * 26800, SDS-PAGE Dioscorea cayenensis subsp. rotundata
dimer 2 * 26800, SDS-PAGE Dioscorea praehensilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.009
-
 2-mercaptoethanol pH 6.5, 30°C Dioscorea cayenensis subsp. rotundata
0.01
-
 2-mercaptoethanol pH 6.5, 30°C Colocasia esculenta
0.01
-
 2-mercaptoethanol pH 6.5, 30°C Dioscorea praehensilis
0.01
-
 2-mercaptoethanol pH 6.5, 30°C Dioscorea alata
0.03
-
 glutathione pH 6.5, 30°C Dioscorea cayenensis subsp. rotundata
0.03
-
 glutathione pH 6.5, 30°C Colocasia esculenta
0.03
-
 glutathione pH 6.5, 30°C Dioscorea alata
0.035
-
 glutathione pH 6.5, 30°C Dioscorea praehensilis
7.9
-
 EDTA pH 6.5, 30°C Dioscorea alata
8.6
-
 EDTA pH 6.5, 30°C Colocasia esculenta
9
-
 EDTA pH 6.5, 30°C Dioscorea praehensilis
10.9
-
 EDTA pH 6.5, 30°C Dioscorea cayenensis subsp. rotundata