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Literature summary for 1.14.18.1 extracted from

  • Batista, K.; Batista, G.; Alves, G.; Fernandes, K.
    Extraction, partial purification and characterization of polyphenol oxidase from Solanum lycocarpum fruits (2014), J. Mol. Catal. B, 102, 211-217 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid uncompetitive inhibition, 70.3% inhibition at 30 mM Solanum lycocarpum
Citric acid uncompetitive inhibition, 74.5% inhibition at 10 mM Solanum lycocarpum
L-cysteine competitive inhibition, 74.5% inhibition at 10 mM Solanum lycocarpum
Sodium metabisulfite uncompetitive inhibition, 70.5% inhibition at 10 mM Solanum lycocarpum
Thiourea uncompetitive inhibition, 46% inhibition at 15 mM Solanum lycocarpum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Solanum lycocarpum
0.15
-
4-methylcatechol pH 6.5, 28°C Solanum lycocarpum
6.47
-
catechol pH 6.5, 28°C Solanum lycocarpum
20.11
-
pyrogallol pH 6.5, 28°C Solanum lycocarpum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 4-methylcatechol + O2 Solanum lycocarpum
-
2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2 Solanum lycocarpum
-
2 1,2-benzoquinone + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycocarpum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, purification procedure is based on freezing precipitation, and a sixfold, partial purification factor is obtained Solanum lycocarpum

Source Tissue

Source Tissue Comment Organism Textmining
fruit unripe fruits present higher activity than ripe for both fresh fruits and dried pulp flours Solanum lycocarpum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
172 U/g dry weight and 215 U/g dry weight in unripe and ripe fruits, respectively Solanum lycocarpum
1.78
-
partially purified enzyme, pH 6.5, 28°C, substrate phenol Solanum lycocarpum
3.82
-
partially purified enzyme, pH 6.5, 28°C, substrate pyrogallol Solanum lycocarpum
20.97
-
partially purified enzyme, pH 6.5, 28°C, substrate catechol Solanum lycocarpum
67.18
-
partially purified enzyme, pH 6.5, 28°C, substrate 4-methylcatechol Solanum lycocarpum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-methylcatechol + O2
-
Solanum lycocarpum 2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
2 4-methylcatechol + O2 best substrate Solanum lycocarpum 2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2
-
Solanum lycocarpum 2 1,2-benzoquinone + 2 H2O
-
?
2 phenol + O2
-
Solanum lycocarpum 2 1,2-benzoquinone + 2 H2O
-
?
additional information the enzyme is classified as a catecholase type polyphenol oxidase Solanum lycocarpum ?
-
?
pyrogallol + O2
-
Solanum lycocarpum ?
-
?

Subunits

Subunits Comment Organism
? x * 47000 + x * 68000, SDS-PAGE Solanum lycocarpum

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
Solanum lycocarpum
PPO
-
Solanum lycocarpum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
Solanum lycocarpum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
results from kinetic and thermodynamic parameters for the thermal inactivation evidence that the partially purified PPO is a biocatalyst, whose inactivation process is related to aggregation of partially unfolded enzyme molecules Solanum lycocarpum
30
-
partially purified enzyme, half-life is 2.71 h Solanum lycocarpum
40
-
partially purified enzyme, half-life is 2.03 h Solanum lycocarpum
50
-
partially purified enzyme, half-life is 1.82 h Solanum lycocarpum
60
-
partially purified enzyme, half-life is 1.51 h Solanum lycocarpum
65
-
partially purified enzyme, half-life is 0.80 h Solanum lycocarpum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Solanum lycocarpum