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Literature summary for 1.14.18.1 extracted from
- Characterization of polyphenol oxidase from Jipicao Yam (2015), J. Food Biochem., 39, 173-178 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Dioscorea polystachya |  |
| Ca2+ | - |
Dioscorea polystachya | |
| Citric acid | - |
Dioscorea polystachya | |
| Cu2+ | slight inhibition at 1 mM | Dioscorea polystachya | |
| EDTA | - |
Dioscorea polystachya | |
| K+ | - |
Dioscorea polystachya | |
| L-cysteine | strong inhibitor | Dioscorea polystachya | |
| Mn2+ | - |
Dioscorea polystachya | |
| Na+ | - |
Dioscorea polystachya | |
| NaHSO3 | strong inhibitor | Dioscorea polystachya | |
| oxalic acid | - |
Dioscorea polystachya | |
| phytic acid | - |
Dioscorea polystachya | |
| Zn2+ | - |
Dioscorea polystachya | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme kinetics | Dioscorea polystachya | |
| 1.5 | - |
chlorogenic acid | pH 6.8, 30°C | Dioscorea polystachya | |
| 4.6 | - |
Coke gallic acid | pH 6.8, 30°C | Dioscorea polystachya | |
| 6.8 | - |
gallic acid | pH 6.8, 30°C | Dioscorea polystachya | |
| 18 | - |
catechol | pH 6.8, 30°C | Dioscorea polystachya | |
| 25.6 | - |
guaiacolum | pH 6.8, 30°C | Dioscorea polystachya | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a copper-containing enzyme | Dioscorea polystachya | |
| Fe3+ | activates | Dioscorea polystachya | |
| Mg2+ | activates | Dioscorea polystachya | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-dopa + O2 | Dioscorea polystachya | - |
2 dopaquinone + 2 H2O | - |
? | |
| L-tyrosine + O2 | Dioscorea polystachya | - |
dopaquinone + H2O | - |
? | |
| additional information | Dioscorea polystachya | PPO is an enzyme concerning the o-hydroxylation of monophenols to o-diphenols acting as cresolase, EC 1.14.18.1, and the oxidation of o-diphenols to o-quinones acting as catecholase, EC 1.10.3.1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dioscorea polystachya | - |
Jipicao yam grown in China | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Dioscorea polystachya | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 catechol + O2 | - |
Dioscorea polystachya | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 2 L-dopa + O2 | - |
Dioscorea polystachya | 2 dopaquinone + 2 H2O | - |
? | |
| chlorogenic acid + O2 | - |
Dioscorea polystachya | ? | - |
? | |
| Coke gallic acid + O2 | - |
Dioscorea polystachya | ? | - |
? | |
| gallic acid + O2 | - |
Dioscorea polystachya | ? | - |
? | |
| guaiacolum + O2 | - |
Dioscorea polystachya | ? | - |
? | |
| L-tyrosine + O2 | - |
Dioscorea polystachya | dopaquinone + H2O | - |
? | |
| additional information | PPO is an enzyme concerning the o-hydroxylation of monophenols to o-diphenols acting as cresolase, EC 1.14.18.1, and the oxidation of o-diphenols to o-quinones acting as catecholase, EC 1.10.3.1 | Dioscorea polystachya | ? | - |
? | |
| additional information | no activity with p-hydroxyphenylalanine | Dioscorea polystachya | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyphenol oxidase | - |
Dioscorea polystachya |
| PPO | - |
Dioscorea polystachya |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Dioscorea polystachya |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | activity range, profile overview | Dioscorea polystachya |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified enzyme, stable up to | Dioscorea polystachya |
| 60 | 70 | purified enzyme, 4 min, loss of 60% activity | Dioscorea polystachya |
| 80 | - |
purified enzyme, 2 min, loss of 90.5% activity | Dioscorea polystachya |
| 90 | 100 | purified enzyme, 1 min, inactivation | Dioscorea polystachya |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Dioscorea polystachya |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | activity range, profile overview | Dioscorea polystachya |
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Release 2023.1 (January 2023)
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