Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons | uncultured bacterium |
DNA and amino acid sequence determination and analysis, sequence comparisons | Streptomyces cyaneofuscatus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilization on electrospun nylon nanofiber membranes | uncultured bacterium |
additional information | enzyme immobilization on electrospun nylon nanofiber membranes | Streptomyces cyaneofuscatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | complete inhibition at 0.1 mM | Streptomyces cyaneofuscatus | |
EDTA | 34% inhibition at 10 mM | Streptomyces cyaneofuscatus | |
L-cysteine | complete inhibition at 0.05 mM | Streptomyces cyaneofuscatus | |
SDS | 78% inhibition at 0.05 mM | Streptomyces cyaneofuscatus | |
Sodium metabisulfite | complete inhibition at 0.1 mM | Streptomyces cyaneofuscatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces cyaneofuscatus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper-containing enzyme | uncultured bacterium | |
Cu2+ | a copper-containing enzyme | Streptomyces cyaneofuscatus | |
additional information | tyrosinase activity is enhanced in general by all the cations tested, apart for Ca2+ and Fe2+, that are poor inhibitors of the enzyme. No inhibition by 1 mM NaCl | Streptomyces cyaneofuscatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | uncultured bacterium | - |
2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | Streptomyces cyaneofuscatus | - |
2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | Streptomyces cyaneofuscatus Ms1 | - |
2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | Streptomyces cyaneofuscatus NRRL B-2570 | - |
2 dopaquinone + 2 H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | inhibits the activity 20-50% at 10-20% v/v | Streptomyces cyaneofuscatus |
Ethanol | inhibits the activity 50% at 10% v/v | Streptomyces cyaneofuscatus |
Methanol | inhibits the activity 50% at 30% v/v | Streptomyces cyaneofuscatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cyaneofuscatus | - |
several strains are isolated from Algerian Sahara soil | - |
Streptomyces cyaneofuscatus | A0A2H4QH72 | - |
- |
Streptomyces cyaneofuscatus Ms1 | - |
several strains are isolated from Algerian Sahara soil | - |
Streptomyces cyaneofuscatus NRRL B-2570 | A0A2H4QH72 | - |
- |
uncultured bacterium | - |
Sahara soil actinobacteria Ms1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 6.8fold by ammonium sulfate fractionation and anion exchange chromatography | Streptomyces cyaneofuscatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.6 | - |
purified enzyme, pH 7.0, temperature not specified in the publication | Streptomyces cyaneofuscatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | - |
uncultured bacterium | 2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | - |
Streptomyces cyaneofuscatus | 2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | - |
Streptomyces cyaneofuscatus Ms1 | 2 dopaquinone + 2 H2O | - |
? | |
2 L-dopa + O2 | - |
Streptomyces cyaneofuscatus NRRL B-2570 | 2 dopaquinone + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Streptomyces cyaneofuscatus |
Synonyms | Comment | Organism |
---|---|---|
melC2 | - |
Streptomyces cyaneofuscatus |
MelC2 tyrosinase | - |
Streptomyces cyaneofuscatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
uncultured bacterium |
55 | - |
- |
Streptomyces cyaneofuscatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 70 | over 60% of maximal activity within this range | uncultured bacterium |
5 | 70 | over 60% of maximal activity within this range | Streptomyces cyaneofuscatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | purified enzyme, 90 min, stable at | uncultured bacterium |
30 | 40 | purified enzyme, 90 min, stable at | Streptomyces cyaneofuscatus |
50 | - |
purified enzyme, loss of 50% activity after 30 min, and of 80% activity after 90 min | uncultured bacterium |
50 | - |
purified enzyme, loss of 50% activity after 30 min, and of 80% activity after 90 min | Streptomyces cyaneofuscatus |
60 | - |
purified enzyme, inactivation within a few minutes | uncultured bacterium |
60 | - |
purified enzyme, inactivation within a few minutes | Streptomyces cyaneofuscatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | uncultured bacterium |
7 | - |
assay at | Streptomyces cyaneofuscatus |
General Information | Comment | Organism |
---|---|---|
evolution | Ms1 genome scaffolds with publicly available Streptomyces genus whole genome sequences show the highest nucleotide identity (99%) with Streptomyces cyaneofuscatus strain NRRL B-2570, genes melC1 and melC2 | uncultured bacterium |