Application | Comment | Organism |
---|---|---|
medicine | tyrosinase purified from Agaricus bisporus is a potential source for medical applications | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.933 | - |
L-Dopa | pH 7.0, 35°C | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | Agaricus bisporus | - |
2 dopaquinone + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 16.36fold by ammonium sulfate fractionation, dialysis, gel filtration, and anion exchange chromatography | Agaricus bisporus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52.19 | - |
purified native enzyme, pH 7.0, 35°C | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | - |
Agaricus bisporus | 2 dopaquinone + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95000, SDS-PAGE | Agaricus bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Agaricus bisporus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | activity range, profile overview | Agaricus bisporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | purified native enzyme, about 35% of tyrosinase activity is still present at 55°C, but activity is lost at 65°C | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Agaricus bisporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, profile overview | Agaricus bisporus |