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Literature summary for 1.14.18.1 extracted from
- A heme peroxidase with a functional role as an L-tyrosine hydroxylase in the biosynthesis of anthramycin (2011), Biochemistry, 50, 8926-8936.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces refuineus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | the enzyme becomes inactivated by hydrogen peroxide during catalysis | Streptomyces refuineus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
L-tyrosine | at pH 8.0 and 37°C | Streptomyces refuineus | |
| 1 | - |
H2O2 | at pH 8.0 and 37°C | Streptomyces refuineus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33600 | - |
1 * 33600, calculated from amino acid sequence | Streptomyces refuineus |
| 37900 | - |
1 * 37900, SDS-PAGE | Streptomyces refuineus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + H2O2 | Streptomyces refuineus | - |
3,4-dihydroxy-L-phenylalanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces refuineus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography, Ni-Sepharose column chromatography, and S-200 gel filtration | Streptomyces refuineus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + H2O2 | - |
Streptomyces refuineus | 3,4-dihydroxy-L-phenylalanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33600, calculated from amino acid sequence | Streptomyces refuineus |
| monomer | 1 * 37900, SDS-PAGE | Streptomyces refuineus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-tyrosine hydroxylase | - |
Streptomyces refuineus |
| Orf13 | - |
Streptomyces refuineus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.576 | - |
L-tyrosine | at pH 8.0 and 37°C | Streptomyces refuineus | |
| 1.5 | - |
H2O2 | at pH 8.0 and 37°C | Streptomyces refuineus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme b | dependent on | Streptomyces refuineus | |
| tetrahydrobiopterin | - |
Streptomyces refuineus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
L-tyrosine | at pH 8.0 and 37°C | Streptomyces refuineus | |
| 1.5 | - |
H2O2 | at pH 8.0 and 37°C | Streptomyces refuineus | |
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Release 2023.1 (January 2023)
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