Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-butyl-3-methylimidazolium tetrafluoroborate | in the presence of 1-butyl-3-methylimidazolium tetrafluoroborate the selectivity of tyrosinase is altered, and the ratio of monophenolase/diphenolase activity increases by up to 5fold | Priestia megaterium | |
ethylammonium nitrate | in the presence of ethylammonium nitrate the selectivity of tyrosinase is altered, and the ratio of monophenolase/diphenolase activity increases by up to 5fold | Priestia megaterium | |
SDS | the addition of SDS at levels of 2-50 mM increases the activity of the enzyme by 2fold towards the natural substrates L-tyrosine and L-Dopa and 15-20fold towards the non-native phenol and catechol | Priestia megaterium |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Priestia megaterium |
Crystallization (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
R209H | the mutant has a 45fold increase in activity towards phenol in the presence of SDS compared to the wild type enzyme | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-methylimidazolium ethyl sulfate | in the presence of 10 or 20% (w/v) 1-ethyl-3-methylimidazolium ethyl sulfate, the activity decreases dramatically and becomes negligible | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | a type 3 copper protein | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Dopa + O2 | Priestia megaterium | - |
L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | Priestia megaterium | - |
L-dopaquinone + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | B2ZB02 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 catechol + O2 | - |
Priestia megaterium | 2 1,2-benzoquinone + 2 H2O | - |
? | |
L-Dopa + O2 | - |
Priestia megaterium | L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | - |
Priestia megaterium | L-dopaquinone + H2O | - |
? | |
phenol + O2 | - |
Priestia megaterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diphenolase | - |
Priestia megaterium |
monophenolase | - |
Priestia megaterium |
tyr | - |
Priestia megaterium |