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Literature summary for 1.14.18.1 extracted from
- Crystallization and preliminary X-ray crystallographic analysis of a bacterial tyrosinase from Bacillus megaterium (2010), Acta Crystallogr. Sect. F, 66, 1101-1103.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Priestia megaterium |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 18% (w/v) PEG 8000, 0.2 M zinc acetate, 0.1 M cacodylic acid pH 5.6-6.1, at 16°C | Priestia megaterium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a type 3 copper enzyme possessing two copper ions in the active site | Priestia megaterium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-bound affinity column chromatography and AEC-HPLC column chromatography | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-DOPA + O2 | - |
Priestia megaterium | dopaquinone + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyr | - |
Priestia megaterium |
| tyrosinase | - |
Priestia megaterium |
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Release 2023.1 (January 2023)
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