Literature summary for 1.14.18.1 extracted from

Fan, T.; Zhang, Y.; Yang, L.; Yang, X.; Jiang, G.; Yu, M.; Cong, R.
Identification and characterization of a hemocyanin-derived phenoloxidase from the crab Charybdis japonica (2009), Comp. Biochem. Physiol. B, 152, 144-149.

Search for more literature for 1.14.18.1

Inhibitors

Inhibitors	Comment	Organism	Structure
1-Phenyl-2-thiourea	the phenoloxidase activity of HdPO is most sensitive to 1-phenyl-2-thiourea, complete inhibition at 5 mM	Charybdis japonica
ascorbic acid	the phenoloxidase activity of HdPO is most sensitive to ascorbic acid, 93% inhibition at 10 mM	Charybdis japonica
benzoic acid	48.8% inhibition at 10 mM	Charybdis japonica
Ca2+	43.9% inhibition at 10 mM	Charybdis japonica
Citric acid	27.9% inhibition at 10 mM	Charybdis japonica
Cu2+	strongly inhibited by Cu2+, 92.7% inhibition at 5 mM	Charybdis japonica
cysteine	the phenoloxidase activity of HdPO is most sensitive to cysteine, complete inhibition at 10 mM	Charybdis japonica
diethyldithiocarbamate	strongly inhibited by diethyldithiocarbamate, 90.2% inhibition at 20 mM	Charybdis japonica
EDTA	strongly inhibited by EDTA, complete inhibition at 10 mM	Charybdis japonica
Mg2+	29.3% inhibition at 10 mM	Charybdis japonica
sodium sulfite	the phenoloxidase activity of HdPO is much sensitive to sodium sulfite, 72.1% inhibition at 10 mM	Charybdis japonica
Thiourea	the phenoloxidase activity of HdPO is much sensitive to thiourea, 83.7% inhibition at 10 mM	Charybdis japonica
Zn2+	strongly inhibited by Zn2+, 80.5% inhibition at 5 mM	Charybdis japonica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.9	-	L-Dopa	in 20 mM Tris-HCl buffer (pH 7.1)	Charybdis japonica
7.33	-	L-tyrosine	in 20 mM Tris-HCl buffer (pH 7.1)	Charybdis japonica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	HdPO is a copper-containing metalloenzyme	Charybdis japonica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	x * 80000 + x * 75000 + n * 70000, SDS-PAGE	Charybdis japonica
75000	-	x * 80000 + x * 75000 + n * 70000, SDS-PAGE	Charybdis japonica
80000	-	x * 80000 + x * 75000 + n * 70000, SDS-PAGE	Charybdis japonica

Organism

Organism	UniProt	Comment	Textmining
Charybdis japonica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE Sepharose column chromatography and Sephacryl S-100 gel filtration	Charybdis japonica

Source Tissue

Source Tissue	Comment	Organism	Textmining
hemolymph	-	Charybdis japonica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tyrosine + L-dopa + O2	-	Charybdis japonica	L-dopa + dopaquinone + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 80000 + x * 75000 + n * 70000, SDS-PAGE	Charybdis japonica

Synonyms

Synonyms	Comment	Organism
Hc-derived phenoloxidase	-	Charybdis japonica
HdPO	-	Charybdis japonica
hemocyanin-derived phenoloxidase	-	Charybdis japonica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Charybdis japonica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Charybdis japonica

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Release 2023.1 (January 2023)