Literature summary for 1.14.18.1 extracted from

Schweikardt, T.; Olivares, C.; Solano, F.; Jaenicke, E.; Garcia-Borron, J.C.; Decker, H.
A three-dimensional model of mammalian tyrosinase active site accounting for loss of function mutations (2007), Pigment Cell Res., 20, 394-401.

Search for more literature for 1.14.18.1

Cloned(Commentary)

Cloned (Comment)	Organism
the wild-type Tyr and mutant constructs are transiently expressed in human embryonic kidney (HEK) 293T cells for functional analysis	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
M374G	potential to delete the enzymatic activity of Tyr. Major effect on the active site: the packing density of this normally rigid environment is significantly lowered when the original amino acid is mutated to the smaller glycine because the missing side chain of G374 can neither anchor the loop nor orient the side chain of H367	Mus musculus
M374G/S375G	Tyr-GG double mutant, potential to delete the enzymatic activity of Tyr. The M374G/S375G mutation, designated Tyr-GG, replaces two residues present in mTyr by the equivalent residues in mTyrp1 and could potentially modify the enzymatic properties of the protein, when compared with wild-type Tyr. Analysis of the behaviour of the individual mutants M374G and S374G indicates that loss of enzymatic activity in Tyr-GG is mostly because of the M374G mutation	Mus musculus
S375G	potential to delete the enzymatic activity of Tyr	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	the active site of the tyrosinase model shows the same structural conformation as in sTyr and ibCO, where two copper ions are coordinated by three histidines each, forming a binuclear type 3 copper site similar to that of the template structure	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	corresponds to the fully deglycosylated protein backbone. Western blot analysis	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P11344	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tyrosine + O2 + AH2	radioactive substrate L-[3,5-3H]-tyrosine, specific activity 50 Ci/mmol	Mus musculus	L-dopa + H2O + A	-	?

Synonyms

Synonyms	Comment	Organism
mTyr	-	Mus musculus
phenoloxidase	-	Mus musculus
tyr	-	Mus musculus
tyrosinase	-	Mus musculus

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Release 2023.1 (January 2023)