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Literature summary for 1.14.18.1 extracted from

  • Flurkey, A.; Cooksey, J.; Reddy, A.; Spoonmore, K.; Rescigno, A.; Inlow, J.; Flurkey, W.H.
    Enzyme, protein, carbohydrate, and phenolic contaminants in commercial tyrosinase preparations: potential problems affecting tyrosinase activity and inhibition studies (2008), J. Agric. Food Chem., 56, 4760-4768.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-hexylresorcinol
-
Agaricus bisporus
ammonium tetramolybdate
-
Agaricus bisporus
esculetin
-
Agaricus bisporus
kojic acid
-
Agaricus bisporus
Methimazole
-
Agaricus bisporus
Salicylhydroxamic acid
-
Agaricus bisporus
tropolone
-
Agaricus bisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
Dopa Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus
0.46
-
Dopa Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) Agaricus bisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Agaricus bisporus

Organism

Organism UniProt Comment Textmining
Agaricus bisporus O42713
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of tyrosinase Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) and Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
498
-
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) (units/mg of powder), WMT and SMT show different levels of tyrosinase activity per milligram of powder Agaricus bisporus
1700
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) (units/mg of powder), WMT and SMT show different levels of tyrosinase activity per milligram of powder Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dopa + 1/2 O2
-
Agaricus bisporus L-dopaquinone + H2O
-
?

Subunits

Subunits Comment Organism
More x * 45000, SDS-PAGE Agaricus bisporus

Synonyms

Synonyms Comment Organism
monophenol, o-diphenol:oxygen oxido-reductase
-
Agaricus bisporus
PPO2
-
Agaricus bisporus
tyrosinase
-
Agaricus bisporus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0008
-
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) Agaricus bisporus Salicylhydroxamic acid
0.0009
-
Sigma mushroom tyrosinase (SMT, partially purified) Agaricus bisporus Salicylhydroxamic acid
0.0012
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus Salicylhydroxamic acid
0.0013
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus tropolone
0.0017
-
Sigma mushroom tyrosinase (SMT, partially purified) Agaricus bisporus tropolone
0.0025
-
Sigma mushroom tyrosinase (SMT, partially purified) Agaricus bisporus 4-hexylresorcinol
0.0075
-
Sigma mushroom tyrosinase (SMT, partially purified) Agaricus bisporus ammonium tetramolybdate
0.018
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus 4-hexylresorcinol
0.025
-
Sigma mushroom tyrosinase (SMT, partially purified) Agaricus bisporus kojic acid
0.028
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus ammonium tetramolybdate
0.03
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus kojic acid
0.04
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus Methimazole
0.045
-
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) Agaricus bisporus kojic acid
0.047
-
Sigma mushroom tyrosinase (SMT, partially purified) Agaricus bisporus Methimazole
0.21
-
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) Agaricus bisporus esculetin
0.225
-
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) Agaricus bisporus esculetin