Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.18.1 extracted from

  • Sutay Kocabas, D.; Bakir, U.; Phillips, S.E.; McPherson, M.J.; Ogel, Z.B.
    Purification, characterization, and identification of a novel bifunctional catalase-phenol oxidase from Scytalidium thermophilum (2008), Appl. Microbiol. Biotechnol., 79, 407-415.
    View publication on PubMed
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
p-coumaric acid 14% residual activity at 0.5 M Mycothermus thermophilus
Salicylhydroxamic acid 36% residual activity at 0.5 M Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 4 * 80000 Mycothermus thermophilus
320000
-
 SDS-PAGE Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
 type culture Humicola insolens, ATCC 16454
-

Purification (Commentary)

Purification (Comment) Organism
HiPrep 16/60 Q XL column chromatography and Sephacryl S-100 gel filtration Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2 the highest oxidase activity is observed against catechol Mycothermus thermophilus 2 1,2-benzoquinone + 2 H2O
-
 ?
caffeic acid + O2 50% activity compared to catechol Mycothermus thermophilus caffeoyl quinone + H2O
-
 ?
L-Dopa + O2 30% activity compared to catechol Mycothermus thermophilus L-dopaquinone + H2O
-
 ?
additional information no activity is detected against L-tyrosine and common laccase substrates such as 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldazine with the exception of weak activity with p-hydroquinone Mycothermus thermophilus ?
-
 ?
p-hydroquinone + O2 2% activity compared to catechol Mycothermus thermophilus ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 80000 Mycothermus thermophilus

Synonyms

Synonyms Comment Organism
catalase-phenol oxidase bifunctional enzyme Mycothermus thermophilus
CATPO
-
 Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Mycothermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 75 relative activity is greater than 70% between 30°C and 75°C for catalase activity, no decrease in activity is observed at 50°C for 2 h, CATPO retains 80% and 45% of its original catalase activity at 60 and 70°C, respectively. Catalase activity is essentially lost after 2 h incubation at 80°C, and at this temperature catalase activity is rapidly reduced with only 30% remaining after 30 min. Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mycothermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 some ca. 80% of catalase activity is retained between pH 6 and 8, while 80% of phenol oxidase activity is retained between pH 7 and 8 with only ca. 25% activity at pH 6 and ca. 50% at pH 8, retains ca. 80% of the initial activity across the pH range after 24 h incubation Mycothermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 most stable at pH 7.0, more than 90% of the initial activity remains after 24 h incubation at pH 7, catalase retains more than 60% of its activity at low (pH 5.5) and high (pH 9) values Mycothermus thermophilus

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains heme Mycothermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Mycothermus thermophilus
-
-
 5