Literature summary for 1.14.18.1 extracted from

Kohashi, P.Y.; Kumagai, T.; Matoba, Y.; Yamamoto, A.; Maruyama, M.; Sugiyama, M.
An efficient method for the overexpression and purification of active tyrosinase from Streptomyces castaneoglobisporus (2004), Protein Expr. Purif., 34, 202-207.

Search for more literature for 1.14.18.1

Cloned(Commentary)

Cloned (Comment)	Organism
overproduction of apotyrosinase (TYRC) and its activator protein (ARF378) in Escherichia coli BL21(DE3)-pLysS. tyrC and arf378 are independently but not polycistronically placed under the control of a T7 promoter in a vector, pET-21a(+). His6-tagged TYRC and His6-tagged ARF378 are simultaneously overproduced in an Escherichia coli strain harboring the plasmid pET-mel2	Streptomyces castaneoglobisporus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
8.1	-	L-Dopa	His6-tagged TYRC	Streptomyces castaneoglobisporus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	the activator protein ORF378 may facilitate the incorporation of Cu(II) into apotyrosinase	Streptomyces castaneoglobisporus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces castaneoglobisporus	-	HUT6202	-
Streptomyces castaneoglobisporus HUT6202	-	HUT6202	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces castaneoglobisporus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1	-	-	Streptomyces castaneoglobisporus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-dopa + 1/2 O2	-	Streptomyces castaneoglobisporus	L-dopaquinone + H2O	-	?
L-dopa + 1/2 O2	-	Streptomyces castaneoglobisporus HUT6202	L-dopaquinone + H2O	-	?

Synonyms

Synonyms	Comment	Organism
tyrosinase	-	Streptomyces castaneoglobisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
880	-	L-Dopa	His6-tagged TYRC	Streptomyces castaneoglobisporus

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Release 2023.1 (January 2023)