Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.1 mM, complete inhibition | Thermomicrobium roseum | |
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | 1 mM, 50% inhibition of L-dopa oxidation | Thermomicrobium roseum | |
Cl- | 1 mM, 35% inhibition of L-dopa oxidation | Thermomicrobium roseum | |
diethyldithiocarbamate | 1 mM, complete inhibition of L-dopa oxidation | Thermomicrobium roseum | |
EDTA | 1 mM, 71% inhibition of L-dopa oxidation | Thermomicrobium roseum | |
glutathione | 1 mM, 86% inhibition of L-dopa oxidation | Thermomicrobium roseum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-Dopa | - |
Thermomicrobium roseum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 50% activation of L-dopa oxidation | Thermomicrobium roseum | |
K+ | 50% activation of L-dopa oxidation | Thermomicrobium roseum | |
Mg2+ | 33% activation of L-dopa oxidation | Thermomicrobium roseum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Thermomicrobium roseum |
90000 | - |
gel filtration | Thermomicrobium roseum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomicrobium roseum | - |
ATCC 27502 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sephacel, Mono Q | Thermomicrobium roseum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.49 | - |
- |
Thermomicrobium roseum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(RS)-catechin + O2 | 83% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
4-hydroxybenzoic acid + O2 + AH2 | 50% of activity with L-dopa | Thermomicrobium roseum | 3,4-dihydroxybenzoic acid + H2O + A | - |
? | |
caffeic acid + 1/2 O2 | 17% of activity with L-dopa | Thermomicrobium roseum | caffeoyl quinone + H2O | - |
? | |
catechol + 1/2 O2 | 125% of activity with L-dopa | Thermomicrobium roseum | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | 108% of activity with L-dopa | Thermomicrobium roseum | chlorogenoquinone + H2O | - |
? | |
D-catechin + O2 | 67% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
DL-epicatechin + O2 | 91% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
dopa + 1/2 O2 | - |
Thermomicrobium roseum | dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | 10% of activity with L-dopa | Thermomicrobium roseum | L-dopa + H2O + A | - |
? | |
o-coumaric acid + O2 | 58% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
orcin + O2 | 42% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
phloroglucin + O2 | 87% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
pyrogallol + O2 | 417% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
resorcinol + O2 | 67% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
tyramine + O2 | 67% of activity with L-dopa | Thermomicrobium roseum | 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Thermomicrobium roseum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Thermomicrobium roseum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | less than 50% activity below pH 7.5 and above pH 10.5, L-dopa oxidation at 50°C | Thermomicrobium roseum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
4°C, 20 h, about 75% loss of activity below | Thermomicrobium roseum |
8.5 | 10 | 4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0 | Thermomicrobium roseum |
11 | - |
4°C, 20 h, about 75% loss of activity above | Thermomicrobium roseum |