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Literature summary for 1.14.18.1 extracted from

  • Ikediobi, C.O.; Obasuyi, H.N.
    Purification and some properties of o-diphenolase from white yam tubers (1982), Phytochemistry, 21, 2815-2820.
No PubMed abstract available
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, complete inhibition Dioscorea cayenensis subsp. rotundata
CN- 1 mM, 96% inhibition Dioscorea cayenensis subsp. rotundata
diethyldithiocarbamate 1 mM, complete inhibition Dioscorea cayenensis subsp. rotundata
DL-dithiothreitol 1 mM, complete inhibition Dioscorea cayenensis subsp. rotundata
iodobenzoic acid 1 mM, 78% inhibition Dioscorea cayenensis subsp. rotundata
L-ascorbic acid 1 mM, complete inhibition of purified enzyme, 20% inhibition of crude enzyme Dioscorea cayenensis subsp. rotundata
L-cysteine 1 mM, complete inhibition Dioscorea cayenensis subsp. rotundata
Metabisulfite 1 mM, complete inhibition Dioscorea cayenensis subsp. rotundata
N3-
-
 Dioscorea cayenensis subsp. rotundata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13
-
 catechol
-
 Dioscorea cayenensis subsp. rotundata

Metals/Ions

Metals/Ions Comment Organism Structure
copper 4 gatom per mol Dioscorea cayenensis subsp. rotundata
copper 0.22% copper, probably 4 copper atoms per enzyme molecule Dioscorea cayenensis subsp. rotundata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
 gel filtration Dioscorea cayenensis subsp. rotundata

Organism

Organism UniProt Comment Textmining
Dioscorea cayenensis subsp. rotundata
-
 white yam from Nigeria
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephadex, Sephadex G-20, DEAE-Sephadex Dioscorea cayenensis subsp. rotundata

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Dioscorea cayenensis subsp. rotundata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 9900 units, 1 unit is defined as the amount of enzyme that causes a change in absorbance of 0.001/min Dioscorea cayenensis subsp. rotundata

Storage Stability

Storage Stability Organism
4°C, 2 weeks, 10% loss of activity Dioscorea cayenensis subsp. rotundata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + 1/2 O2
-
 Dioscorea cayenensis subsp. rotundata 1,2-benzoquinone + H2O
-
 ?
chlorogenic acid + O2
-
 Dioscorea cayenensis subsp. rotundata chlorogenoquinone + H2O
-
 ?
dopa + 1/2 O2
-
 Dioscorea cayenensis subsp. rotundata dopaquinone + H2O
-
 ?
pyrogallol + O2
-
 Dioscorea cayenensis subsp. rotundata ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Dioscorea cayenensis subsp. rotundata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, stable Dioscorea cayenensis subsp. rotundata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Dioscorea cayenensis subsp. rotundata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 L-cysteine
-
 Dioscorea cayenensis subsp. rotundata
0.2
-
 CN-
-
 Dioscorea cayenensis subsp. rotundata
0.5
-
 2-mercaptoethanol
-
 Dioscorea cayenensis subsp. rotundata
1.8
-
 N3-
-
 Dioscorea cayenensis subsp. rotundata