Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.18.1 extracted from

  • S¸derh§ll, I.; S¸derh§ll, K.
    Purification of prophenol oxidase from Daucus carota cell cultures (1989), Phytochemistry, 28, 1805-1808.
No PubMed abstract available
Search for more literature for 1.14.18.1

Activating Compound

Activating Compound Comment Organism Structure
Trypsin activation of proenzyme Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ inactive proenzyme is maximally activated by 1 mM Ca2+ Daucus carota
Mn2+ activation of proenzyme Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000, proenzyme, SDS-PAGE Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
 carrot
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation of proenzyme Daucus carota

Purification (Commentary)

Purification (Comment) Organism
proenzyme, ammonim sulfate, DEAE-cellulose, Cu2+-Sepharose, Sephacryl S-200 Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1064.0 DELTA absorbance 490 nm/min/mg Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
 Daucus carota 4-methyl-o-benzoquinone + H2O
-
 ?
dopa + 1/2 O2
-
 Daucus carota dopaquinone + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, proenzyme, SDS-PAGE Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 7.2
-
 Daucus carota