The hydroxylation step in the biosynthetic pathway leading from norcolaurine to reticuline
Loeffler, S.; Zenk, M.H.; Phytochemistry 29, 3499-3503 (1990)
No PubMed abstract available
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
azide
12 mM, 50% inhibition
Berberis stolonifera
Bathocuproine
-
Berberis stolonifera
CN-
1 mM, complete inhibition
Berberis stolonifera
diethyldithiocarbamate
0.3 mM, complete inhibition
Berberis stolonifera
dipicolinic acid
-
Berberis stolonifera
EDTA
no inhibition EDTA
Berberis stolonifera
Thiourea
13 mM, 50% inhibition
Berberis stolonifera
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, SDS-PAGE
Berberis stolonifera
60000
-
gel filtration
Berberis stolonifera
Organism
Organism
UniProt
Commentary
Textmining
Berberis stolonifera
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing
Berberis stolonifera
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.065
-
with tyrosine
Berberis stolonifera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-coclaurine + ascorbate + O2
-
636344
Berberis stolonifera
(R)-3-hydroxycoclaurine + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
(S)-coclaurine + ascorbate + O2
-
636344
Berberis stolonifera
(S)-3-hydroxycoclaurine + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
4-methylcatechol + ascorbate + O2
-
636344
Berberis stolonifera
4-methyl-o-benzoquinone + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
L-tyrosine + ascorbate + O2
-
636344
Berberis stolonifera
L-dopa + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
N-methylcoclaurine + ascorbate + O2
-
636344
Berberis stolonifera
3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
tyramine + ascorbate + O2
-
636344
Berberis stolonifera
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 36000, SDS-PAGE
Berberis stolonifera
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
30
-
Berberis stolonifera
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Berberis stolonifera
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
7.5
50% of maximal activity at pH 4.5 and pH 7.5
Berberis stolonifera
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
azide
12 mM, 50% inhibition
Berberis stolonifera
Bathocuproine
-
Berberis stolonifera
CN-
1 mM, complete inhibition
Berberis stolonifera
diethyldithiocarbamate
0.3 mM, complete inhibition
Berberis stolonifera
dipicolinic acid
-
Berberis stolonifera
EDTA
no inhibition EDTA
Berberis stolonifera
Thiourea
13 mM, 50% inhibition
Berberis stolonifera
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, SDS-PAGE
Berberis stolonifera
60000
-
gel filtration
Berberis stolonifera
Purification (Commentary) (protein specific)
Commentary
Organism
Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing
Berberis stolonifera
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.065
-
with tyrosine
Berberis stolonifera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-coclaurine + ascorbate + O2
-
636344
Berberis stolonifera
(R)-3-hydroxycoclaurine + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
(S)-coclaurine + ascorbate + O2
-
636344
Berberis stolonifera
(S)-3-hydroxycoclaurine + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
4-methylcatechol + ascorbate + O2
-
636344
Berberis stolonifera
4-methyl-o-benzoquinone + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
L-tyrosine + ascorbate + O2
-
636344
Berberis stolonifera
L-dopa + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
N-methylcoclaurine + ascorbate + O2
-
636344
Berberis stolonifera
3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
tyramine + ascorbate + O2
-
636344
Berberis stolonifera
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O
-
636344
Berberis stolonifera
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 36000, SDS-PAGE
Berberis stolonifera
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
30
-
Berberis stolonifera
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Berberis stolonifera
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
7.5
50% of maximal activity at pH 4.5 and pH 7.5
Berberis stolonifera
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636344
Loeffler
-
The hydroxylation step in the ...
Berberis stolonifera
Phytochemistry
29
3499-3503
1990
-
-
-
-
-
-
7
-
-
-
2
-
-
1
-
-
1
-
-
-
1
-
6
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
2
-
-
-
-
1
-
-
1
-
6
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-