Data extracted from this reference:
Inhibitors
azide
12 mM, 50% inhibition
Berberis stolonifera
Bathocuproine
Berberis stolonifera
CN-
1 mM, complete inhibition
Berberis stolonifera
diethyldithiocarbamate
0.3 mM, complete inhibition
Berberis stolonifera
dipicolinic acid
Berberis stolonifera
EDTA
no inhibition EDTA
Berberis stolonifera
Thiourea
13 mM, 50% inhibition
Berberis stolonifera
Molecular Weight [Da]
36000
2 * 36000, SDS-PAGE
Berberis stolonifera
60000
gel filtration
Berberis stolonifera
Organism
Purification (Commentary)
Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing
Berberis stolonifera
Specific Activity [micromol/min/mg]
0.065
with tyrosine
Berberis stolonifera
Substrates and Products (Substrate)
(R)-coclaurine + ascorbate + O2
636344
Berberis stolonifera
(R)-3-hydroxycoclaurine + dehydroascorbate + H2O
636344
Berberis stolonifera
?
(S)-coclaurine + ascorbate + O2
636344
Berberis stolonifera
(S)-3-hydroxycoclaurine + dehydroascorbate + H2O
636344
Berberis stolonifera
?
4-methylcatechol + ascorbate + O2
636344
Berberis stolonifera
4-methyl-o-benzoquinone + dehydroascorbate + H2O
636344
Berberis stolonifera
?
L-tyrosine + ascorbate + O2
636344
Berberis stolonifera
L-dopa + dehydroascorbate + H2O
636344
Berberis stolonifera
?
N-methylcoclaurine + ascorbate + O2
636344
Berberis stolonifera
3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
636344
Berberis stolonifera
?
tyramine + ascorbate + O2
636344
Berberis stolonifera
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O
636344
Berberis stolonifera
?
Subunits
dimer
2 * 36000, SDS-PAGE
Berberis stolonifera
Temperature Optimum [°C]
20
30
Berberis stolonifera
pH Optimum
pH Range
4.5
7.5
50% of maximal activity at pH 4.5 and pH 7.5
Berberis stolonifera
Inhibitors (protein specific)
azide
12 mM, 50% inhibition
Berberis stolonifera
Bathocuproine
Berberis stolonifera
CN-
1 mM, complete inhibition
Berberis stolonifera
diethyldithiocarbamate
0.3 mM, complete inhibition
Berberis stolonifera
dipicolinic acid
Berberis stolonifera
EDTA
no inhibition EDTA
Berberis stolonifera
Thiourea
13 mM, 50% inhibition
Berberis stolonifera
Molecular Weight [Da] (protein specific)
36000
2 * 36000, SDS-PAGE
Berberis stolonifera
60000
gel filtration
Berberis stolonifera
Purification (Commentary) (protein specific)
Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing
Berberis stolonifera
Specific Activity [micromol/min/mg] (protein specific)
0.065
with tyrosine
Berberis stolonifera
Substrates and Products (Substrate) (protein specific)
(R)-coclaurine + ascorbate + O2
636344
Berberis stolonifera
(R)-3-hydroxycoclaurine + dehydroascorbate + H2O
636344
Berberis stolonifera
?
(S)-coclaurine + ascorbate + O2
636344
Berberis stolonifera
(S)-3-hydroxycoclaurine + dehydroascorbate + H2O
636344
Berberis stolonifera
?
4-methylcatechol + ascorbate + O2
636344
Berberis stolonifera
4-methyl-o-benzoquinone + dehydroascorbate + H2O
636344
Berberis stolonifera
?
L-tyrosine + ascorbate + O2
636344
Berberis stolonifera
L-dopa + dehydroascorbate + H2O
636344
Berberis stolonifera
?
N-methylcoclaurine + ascorbate + O2
636344
Berberis stolonifera
3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
636344
Berberis stolonifera
?
tyramine + ascorbate + O2
636344
Berberis stolonifera
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O
636344
Berberis stolonifera
?
Subunits (protein specific)
dimer
2 * 36000, SDS-PAGE
Berberis stolonifera
Temperature Optimum [°C] (protein specific)
20
30
Berberis stolonifera
pH Optimum (protein specific)
pH Range (protein specific)
4.5
7.5
50% of maximal activity at pH 4.5 and pH 7.5
Berberis stolonifera
Other publictions for EC
636344
Loeffler
-
The hydroxylation step in the ...
Berberis stolonifera
Phytochemistry
29
3499-3503
1990
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7
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6
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1
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1
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6
1
1
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1
1
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