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Literature summary for 1.14.17.4 extracted from

  • Seo, Y.S.; Yoo, A.; Jung, J.; Sung, S.K.; Yang, D.R.; Kim, W.T.; Lee, W.
    The active site and substrate-binding mode of 1-aminocyclopropane-1-carboxylate oxidase determined by site-directed mutagenesis and comparative modeling studies (2004), Biochem. J., 380, 339-346.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.17.4

Protein Variants

Protein Variants Comment Organism
R244A enzymic activity about 20% of wild-type Malus domestica
R244G enzymic activity about 20% of wild-type Malus domestica
R244K enzymic activity about 20% of wild-type Malus domestica
S246A enzymic activity about 50% of wild-type Malus domestica
S246F enzymic activity less than 5% of wild-type Malus domestica
S246G enzymic activity about 50% of wild-type Malus domestica
S246T enzymic activity less than 5% of wild-type Malus domestica
S246Y enzymic activity less than 5% of wild-type Malus domestica
S257A KM-value similar to wild-type Malus domestica
T157A enzymic activity about 40% of wild-type Malus domestica
V159G enzymic activity about 30% of wild-type Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 ascorbate mutant S246A, pH 7.4, 30°C Malus domestica
0.34
-
 ascorbate mutant E109D, pH 7.4, 30°C Malus domestica
0.36
-
 ascorbate mutant S257A, pH 7.4, 30°C Malus domestica
0.38
-
 ascorbate wild-type, pH 7.4, 30°C Malus domestica
0.82
-
 ascorbate mutant T157A, pH 7.4, 30°C Malus domestica
0.82
-
 ascorbate mutant V159G, pH 7.4, 30°C Malus domestica
0.92
-
 ascorbate mutant S246G, pH 7.4, 30°C Malus domestica
0.99
-
 ascorbate mutant R244A, pH 7.4, 30°C Malus domestica
1.2
-
 ascorbate mutant R244K, pH 7.4, 30°C Malus domestica
1.25
-
 ascorbate mutant R244G, pH 7.4, 30°C Malus domestica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ together with cosubstrate ascorbate, located in 2-His-1-carboxylate facial triad binding pocket Malus domestica

Organism

Organism UniProt Comment Textmining
Malus domestica
-
 Borkh.
-

Reaction

Reaction Comment Organism Reaction ID
1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O modelling studies, catalytic mechanism Malus domestica
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-amino-2-ethylcyclopropane-1-carboxylate + L-ascorbate + O2 + HCO3-
-
 Malus domestica 1-butene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?