Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-phenylbut-3-enoic acid | PBA | Homo sapiens | |
diethyldithiocarbamate | DDC | Homo sapiens | |
Disulfiram | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
Trinitrophenyl-D-Tyr-Val-Gly | PC-3 TIS media | Homo sapiens | |
0.0068 | - |
Trinitrophenyl-D-Tyr-Val-Gly | DU-145 TIS media | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
extracellular | - |
Homo sapiens | - |
- |
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
PAM, determined by SDS-PAGE and Western blot analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PHM replaces the pro-(S) hydrogen of the carboxy-terminal glycine with a hydroxyl group to form an alpha-hydroxyglycine intermediate | ? | - |
? | |
peptidylglycine + ascorbate + O2 | Homo sapiens | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cell extracts are prepared | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CaP cell line | - |
Homo sapiens | - |
DU-145 cell | - |
Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PHM replaces the pro-(S) hydrogen of the carboxy-terminal glycine with a hydroxyl group to form an alpha-hydroxyglycine intermediate | Homo sapiens | ? | - |
? | |
peptidylglycine + ascorbate + O2 | - |
Homo sapiens | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
trinitrophenyl-D-Tyr-Val-Gly + ascorbate + O2 | activity assay | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAM | PAM consists of O2-sensitive PHM and peptidyl-alpha-hydroxyglycine alpha-amidating lyase, PAL, activities | Homo sapiens |
peptidylglycine alpha-amidating monooxygenase | - |
Homo sapiens |
peptidylglycine alpha-hydroxylating monooxygenase | - |
Homo sapiens |
PHM | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00051 | - |
Disulfiram | DU-145 TIS media | Homo sapiens | |
0.00065 | - |
Disulfiram | PC-3 TIS media | Homo sapiens | |
0.00113 | - |
diethyldithiocarbamate | DU-145 TIS media | Homo sapiens | |
0.00119 | - |
diethyldithiocarbamate | PC-3 TIS media | Homo sapiens | |
0.0034 | - |
4-phenylbut-3-enoic acid | PC-3 TIS media | Homo sapiens | |
0.0057 | - |
4-phenylbut-3-enoic acid | DU-145 TIS media | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000053 | - |
PC-3 TIS media | Homo sapiens | diethyldithiocarbamate | |
0.000096 | - |
PC-3 TIS media | Homo sapiens | 4-phenylbut-3-enoic acid | |
0.00011 | - |
DU-145 TIS media | Homo sapiens | diethyldithiocarbamate | |
0.00068 | - |
DU-145 TIS media | Homo sapiens | 4-phenylbut-3-enoic acid |