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Literature summary for 1.14.17.3 extracted from

  • Bauman, A.T.; Yukl, E.T.; Alkevich, K.; McCormack, A.L.; Blackburn, N.J.
    The hydrogen peroxide reactivity of peptidylglycine monooxygenase supports a Cu(II)-superoxo catalytic intermediate (2006), J. Biol. Chem., 281, 4190-4198.
    View publication on PubMed
Search for more literature for 1.14.17.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO DG44 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H172A mutation in the copper center of domain one, no activity in presence of H2O2 Rattus norvegicus
H242A mutation in the copper center of domain two, no activity in presence of H2O2 Rattus norvegicus
additional information only the residues 42-356 carrying the monoogenase domain was expressed Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
 dansyl-D-Tyr-Val-Gly 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, ascorbate Rattus norvegicus
0.8
-
 dansyl-D-Tyr-Val-Gly 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, 1 mM H2O2 Rattus norvegicus
2.78
-
 H2O2 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, H2O2 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ requirred Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two chromatoography steps Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
dansyl-D-Tyr-Val-Gly + H2O2 peptidylglycine monooxygenase is able to catalyze the hydroxylation of peptidylglycine substrates starting from the oxidized enzyme and using hydrogen peroxide as the only source of oxygen Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + H2O
-
 ?
N-dansyl-L-Tyr-L-Val-Gly + ascorbate + O2
-
 Rattus norvegicus N-dansyl-L-Tyr-L-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
peptidylglycine alpha-hydroxylating monooxygenase
-
 Rattus norvegicus
peptidylglycine monooxygenase
-
 Rattus norvegicus
PHMcc
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, H2O2 Rattus norvegicus
9.2
-
 N-dansyl-L-Tyr-L-Val-Gly 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, ascorbate Rattus norvegicus