Cloned (Comment) | Organism |
---|---|
single copy gene, alternative splicing generates several forms of enzyme mRNA, overview | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Phenyl-3-butenoic acid | binds to the lumenal side of the enzyme where the peptidylglycine alpha-hydroxylating PHM activity is localized | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | transmembrane protein with lumenal and cytoplasmic parts, overview, abundant in atrial secretory vesicles | Rattus norvegicus | 16020 | - |
vesicle | from atrial myocytes, enzyme is recruited by aggregates of pro-atrial natriuretic peptide proANP, N-terminally deleted proANP is inhibited in recruiting the enzyme | Rattus norvegicus | 31982 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 125000, PAM-1, SDS-PAGE, x * 110000, PAM-2, SDS-PAGE, x * 90000, PAM-3, SDS-PAGE | Rattus norvegicus |
110000 | - |
x * 125000, PAM-1, SDS-PAGE, x * 110000, PAM-2, SDS-PAGE, x * 90000, PAM-3, SDS-PAGE | Rattus norvegicus |
125000 | - |
x * 125000, PAM-1, SDS-PAGE, x * 110000, PAM-2, SDS-PAGE, x * 90000, PAM-3, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | enzyme might be involved in secretory vesicle budding and fusion in the cardiac ANP-secretory pathway | ? | - |
? | |
peptidylglycine + ascorbate + O2 | Rattus norvegicus | peptidylglycine alpha-hydroxylating monooxygenase reaction | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley, at least 3 different splicing variants PAM-1, PAM-2, and PAM-3 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O | bifunctional enzyme showing peptidylglycine alpha-hydroxylating monooxygenase, EC 1.14.17.3, and peptidylamidoglycolate lyase, PAL, EC 4.3.2.5, activities, the enzyme possesses 2 catalytic domains | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
atrial appendage | from neonatal rats | Rattus norvegicus | - |
atrium | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
myocyte | atrial myocyte | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme might be involved in secretory vesicle budding and fusion in the cardiac ANP-secretory pathway | Rattus norvegicus | ? | - |
? | |
peptidylglycine + ascorbate + O2 | peptidylglycine alpha-hydroxylating monooxygenase reaction | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 125000, PAM-1, SDS-PAGE, x * 110000, PAM-2, SDS-PAGE, x * 90000, PAM-3, SDS-PAGE | Rattus norvegicus |
More | enzyme PAM-1, the longest transcript, is composed of, at the lumenal side, the peptidylglycine alpha-hydroxylating monooxygenase PHM catalytic domain, a noncatalytic domain from exon A, the peptidyl-alpha-hydroxyglacine alpha-amidating lyase PAL catalytic domain, a transmembrane domain, and a C-terminal cytoplasmic domain, deletion of exon A yields PAM-2, further deletion of the transmembrane domain yields soluble PAM-3 within the vesicle lumen | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PAM | - |
Rattus norvegicus |
peptidyl-glycine alpha-amidating monooxygenase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Rattus norvegicus |