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Literature summary for 1.14.17.3 extracted from

  • Glembotski, C.C.; Eipper, B.A.; Mains, R.E.
    Characterization of a peptide alpha-amidation activity from rat anterior pituitary (1984), J. Biol. Chem., 259, 6385-6392.
    View publication on PubMed
Search for more literature for 1.14.17.3

Inhibitors

Inhibitors Comment Organism Structure
adrenocorticotrophic hormone(1-10)
-
 Rattus norvegicus
alpha-N-acetyl-adrenocorticotrophic hormone(1-14)
-
 Rattus norvegicus
endorphin(51-61)NH2 i.e. pro-adrenocorticotrophic hormone(1-10) Rattus norvegicus
endorphins and proendorphins Rattus norvegicus
peptides especially those with COOH-terminal glycine residues Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
secretory granule
-
 Rattus norvegicus 30141
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ Cu2+ strongly stimulates activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pituitary gland anterior Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 very low activity in crude subcellular preparations Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-acetyl-adrenocorticotrophic hormone(1-14) + ascorbate + O2 inhibitory substrate Rattus norvegicus alpha-N-acetyl-adrenocorticotrophic hormone(1-13)-2-hydroxyglycine + dehydroascorbate + H2O
-
 r
D-Tyr-Val-Gly + ascorbate + O2 synthetic peptid Rattus norvegicus D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
monoiodo-D-Tyr-Val-Gly + ascorbate + O2 synthetic peptid Rattus norvegicus monoiodo-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
additional information specificity Rattus norvegicus ?
-
 ?
additional information endorphins are inhibitory substrates Rattus norvegicus ?
-
 ?
peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2 requirement for O2 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Rattus norvegicus
7
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 about 70% of activity maximum at pH 6.0 and 7.5 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ascorbate dependent on Rattus norvegicus
FAD slight activity with Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
 endorphin(51-61)NH2
-
 Rattus norvegicus
0.001
-
 alpha-N-acetyl-adrenocorticotrophic hormone(1-14)
-
 Rattus norvegicus
0.005
-
 adrenocorticotrophic hormone (1-10)
-
 Rattus norvegicus