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Literature summary for 1.14.17.3 extracted from

  • Merkler, D.J.; Young, S.D.
    Recombinant type A rat 75-kDa alpha-amidating enzyme catalyzes the conversion of glycine-extended peptides to peptide amides via an alpha-hydroxyglycine intermediate (1991), Arch. Biochem. Biophys., 289, 192-196.
    View publication on PubMed
Search for more literature for 1.14.17.3

Cloned(Commentary)

Cloned (Comment) Organism
truncated type A enzyme, expression in mouse C127 cells Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information different assay methods Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ Cu2+ is absolutely required for optimal activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 recombinant form B, gel filtration and SDS-PAGE Rattus norvegicus
75000
-
 recombinant form A, gel filtration and SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 2 forms: type A and B
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from mouse cells Rattus norvegicus
type A and B Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-D-Tyr-Val-Gly + ascorbate + O2 stereospecific Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, type A, SDS-PAGE Rattus norvegicus
monomer 1 * 43000, type B, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
More the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ascorbate dependent on Rattus norvegicus