Cloned (Comment) | Organism |
---|---|
truncated type A enzyme, expression in mouse C127 cells | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | different assay methods | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | Cu2+ is absolutely required for optimal activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
recombinant form B, gel filtration and SDS-PAGE | Rattus norvegicus |
75000 | - |
recombinant form A, gel filtration and SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
2 forms: type A and B | - |
Purification (Comment) | Organism |
---|---|
recombinant from mouse cells | Rattus norvegicus |
type A and B | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dansyl-D-Tyr-Val-Gly + ascorbate + O2 | stereospecific | Rattus norvegicus | dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, type A, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 43000, type B, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | dependent on | Rattus norvegicus |