Cloned (Comment) | Organism |
---|---|
gene dbh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis | Penaeus vannamei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | LvDBH expression is silenced in thoracic ganglia, haemocytes and hepatopancreas of shrimp by treatment with LvDBH-dsRNA for 3 days, a decrease of DBH contents in haemocytes accompanied by decreased levels of norepinephrine and dopamine in hemolymph are observed | Penaeus vannamei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Disulfiram | pharmaceutical inhibition of enzyme DBH | Penaeus vannamei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dopamine + ascorbate + O2 | Penaeus vannamei | - |
noradrenaline + dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus vannamei | A0A1I9LDK0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation | Penaeus vannamei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemocyte | - |
Penaeus vannamei | - |
hepatopancreas | - |
Penaeus vannamei | - |
additional information | determination of tissue distribution of enzyme expression by quantitative real-time PCR enzyme expression analysis | Penaeus vannamei | - |
thoracic ganglion | - |
Penaeus vannamei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dopamine + ascorbate + O2 | - |
Penaeus vannamei | noradrenaline + dehydroascorbate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains a DOMON domain, a Cu2_monooxygen domain, and three glycosylation sites | Penaeus vannamei |
Synonyms | Comment | Organism |
---|---|---|
DBH | - |
Penaeus vannamei |
dopamine beta-hydroxylase | - |
Penaeus vannamei |
LvDBH | - |
Penaeus vannamei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Penaeus vannamei |
Organism | Comment | Expression |
---|---|---|
Penaeus vannamei | LvDBH expression is silenced in thoracic ganglia, haemocytes and hepatopancreas of shrimp by treatment with LvDBH-dsRNA for 3 days, a decrease of DBH contents in haemocytes accompanied by decreased levels of norepinephrine and dopamine in hemolymph are observed. LvDBH expressions in thoracic ganglia, haemocytes, and hepatopancreas of shrimp transferred from 28°C to 20°C for 30 min are significant higher than those held at 28°C, hypothermal stress reduces the expression of LvDBH | down |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the copper type II, ascorbate-dependent monooxygenases | Penaeus vannamei |
malfunction | enzyme LvDBH inhibited or silenced in haemocytes by disulfiram and LvDBH-dsRNA, repectively, results in impaired synthesis of norepinephrine from dopamine in hemolymph | Penaeus vannamei |
physiological function | dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to norepinephrine in the biosynthesis of catecholamines. The enzyme plays a critical role in catecholamine synthesis of neuroendocrine regulatory network, and is suggested to be involved in the immunoendocrine responses of invertebrate against bacterial challenge | Penaeus vannamei |