Cloned (Comment) | Organism |
---|---|
TbetaM expressed in Drosophila Schneider 2 (S2) cells | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | very strong substrate inhibition that is dependent on the level of the co-substrate, O2, and reductant as well as substrate deuteration, substrate inhibition ameliorated at very high ascorbate levels | Drosophila melanogaster | |
tyramine | rate of ascorbate reduction of the E-Cu(II) form of TbetaM is also reduced at high tyramine | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0252 | - |
tyramine | at 2 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
0.0876 | - |
tyramine | at 10 mM ascorbate, 0.212 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.088 | - |
tyramine | at 10 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
0.0906 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.907 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.094 | - |
tyramine | at 10 mM ascorbate, 0.058 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.096 | - |
tyramine | at 4 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
0.0976 | - |
tyramine | at 10 mM ascorbate, 1.04 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.098 | - |
tyramine | at 10 mM ascorbate, 0.512 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.113 | - |
tyramine | at 10 mM ascorbate, 0.103 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.145 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.395 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.159 | - |
tyramine | at 10 mM ascorbate, 0.885 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.177 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.119 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.178 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.215 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.188 | - |
tyramine | at 50 mM ascorbate, 0.059 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.193 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 1.039 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.203 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.587 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.215 | - |
tyramine | at 50 mM ascorbate, 0.099 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.23 | - |
tyramine | at 50 mM ascorbate, 1.04 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.231 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.056 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.233 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.907 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.235 | - |
tyramine | at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.235 | - |
tyramine | at 50 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
0.242 | - |
tyramine | at 50 mM ascorbate, 0.395 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.245 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.256 | - |
tyramine | at 50 mM ascorbate, 0.608 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.257 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.110 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.265 | - |
tyramine | at 200 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
0.316 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.598 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.328 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.416 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.341 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.063 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.367 | - |
tyramine | at 100 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
0.387 | - |
tyramine | at 50 mM ascorbate, 0.854 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
0.93 | - |
ascorbate | at 0.05 mM tyramine, pH 6 and 35°C | Drosophila melanogaster | |
1.98 | - |
ascorbate | at 0.1 mM tyramine, pH 6 and 35°C | Drosophila melanogaster | |
5.6 | - |
ascorbate | at 0.3 mM tyramine, pH 6 and 35°C | Drosophila melanogaster | |
16 | - |
ascorbate | at 0.7 mM tyramine, pH 6 and 35°C | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | tyramine can bind to either the Cu(I) or Cu(II) forms of TbetaM | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by anion exchange and His tag affinity chromatography, and gel filtration | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
S2 cell | - |
Drosophila melanogaster | - |
Storage Stability | Organism |
---|---|
-80°C | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta,beta-dideuterated tyramine + ascorbate + O2 | - |
Drosophila melanogaster | octopamine + dehydroascorbate + H2O | - |
? | |
tyramine + ascorbate + O2 | - |
Drosophila melanogaster | octopamine + dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TBetaM | - |
Drosophila melanogaster |
tyramine beta-monooxygenase | - |
Drosophila melanogaster |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
ascorbate | at 0.05 mM tyramine, pH 6 and 35°C | Drosophila melanogaster | |
0.0018 | - |
ascorbate | at 0.1 mM tyramine, pH 6 and 35°C | Drosophila melanogaster | |
0.98 | - |
tyramine | at 10 mM ascorbate, 0.885 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
1.23 | - |
tyramine | at 10 mM ascorbate, 1.04 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
1.25 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.587 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
1.3 | - |
tyramine | at 4 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
1.57 | - |
tyramine | at 50 mM ascorbate, 0.854 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
1.9 | - |
tyramine | at 50 mM ascorbate, 0.608 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.907 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.03 | - |
tyramine | at 50 mM ascorbate, 1.04 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.07 | - |
tyramine | at 10 mM ascorbate, 0.512 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.1 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.215 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.1 | - |
tyramine | at 2 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
2.4 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.395 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.46 | - |
tyramine | at 50 mM ascorbate, 0.395 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.8 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.119 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.8 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.416 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
2.86 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 1.039 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
3.3 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.598 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
3.33 | - |
tyramine | at 10 mM ascorbate, 0.103 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
3.35 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.056 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
3.5 | - |
tyramine | at 10 mM ascorbate, 0.212 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
3.5 | - |
tyramine | at 10 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
3.6 | - |
tyramine | at 100 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
3.97 | - |
tyramine | at 50 mM ascorbate, 0.099 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
4 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.907 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
4.15 | - |
tyramine | at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
4.15 | - |
tyramine | at 50 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster | |
6 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.110 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
7.4 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.063 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
7.8 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
7.82 | - |
tyramine | at 10 mM ascorbate, 0.058 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
10.2 | - |
tyramine | at 50 mM ascorbate, 0.059 mM O2, pH 6 and 35°C | Drosophila melanogaster | |
10.3 | - |
tyramine | at 200 mM ascorbate, pH 6 and 35°C | Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
physiological function | tyramine beta-monooxygenase is homologous to mammalian dopamine beta-monooxygenase, tighter regulation of neurotransmitter levels by the insect enzyme than in the mammalian homologue | Drosophila sp. (in: flies) |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
ascorbate | at 0.7 mM tyramine, pH 6 and 35°C | Drosophila sp. (in: flies) | |
0.8 | - |
ascorbate | at 0.3 mM tyramine, pH 6 and 35°C | Drosophila sp. (in: flies) | |
1.14 | - |
ascorbate | at 0.1 mM tyramine, pH 6 and 35°C | Drosophila sp. (in: flies) | |
1.38 | - |
ascorbate | at 0.05 mM tyramine, pH 6 and 35°C | Drosophila sp. (in: flies) | |
4.95 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.056 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
5.8 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.063 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
9.6 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.110 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
10.9 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.119 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
13.5 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
14.3 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.215 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
15.4 | - |
tyramine | at 50 mM ascorbate, 0.059 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
19.4 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.395 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
19.4 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.416 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
20.1 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.587 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
20.7 | - |
tyramine | at 10 mM ascorbate, 0.058 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
24.6 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.598 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
25.5 | - |
tyramine | at 10 mM ascorbate, 0.103 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
27.2 | - |
tyramine | at 50 mM ascorbate, 0.099 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
29.2 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 0.907 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
31.9 | - |
tyramine | at 200 mM ascorbate, pH 6 and 35°C | Drosophila sp. (in: flies) | |
32.4 | - |
tyramine | at 100 mM ascorbate, pH 6 and 35°C | Drosophila sp. (in: flies) | |
33 | - |
tyramine | at 4 mM ascorbate, pH 6 and 35°C | Drosophila sp. (in: flies) | |
37.8 | - |
tyramine | at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
37.8 | - |
tyramine | at 50 mM ascorbate, pH 6 and 35°C | Drosophila sp. (in: flies) | |
38.6 | - |
beta,beta-dideuterated tyramine | at 50 mM ascorbate, 1.039 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
39.4 | - |
beta,beta-dideuterated tyramine | at 10 mM ascorbate, 0.907 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
44.7 | - |
tyramine | at 10 mM ascorbate, 0.212 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
45 | - |
tyramine | at 10 mM ascorbate, pH 6 and 35°C | Drosophila sp. (in: flies) | |
47.7 | - |
tyramine | at 50 mM ascorbate, 0.395 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
48.2 | - |
tyramine | at 50 mM ascorbate, 0.854 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
52 | - |
tyramine | at 2 mM ascorbate, pH 6 and 35°C | Drosophila sp. (in: flies) | |
58.1 | - |
tyramine | at 10 mM ascorbate, 0.512 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
58.6 | - |
tyramine | at 50 mM ascorbate, 0.608 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
61.7 | - |
tyramine | at 10 mM ascorbate, 0.885 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
70 | - |
tyramine | at 50 mM ascorbate, 1.04 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) | |
74.3 | - |
tyramine | at 10 mM ascorbate, 1.04 mM O2, pH 6 and 35°C | Drosophila sp. (in: flies) |