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Literature summary for 1.14.16.5 extracted from

  • Kosar-Hashemi, B.; Armarego, W.L.F.
    A convenient spectrophotometric method for measuring the kinetic parameters of glyceryl-ether monooxygenase (EC 1.14.16.5) (1993), Biol. Chem. Hoppe-Seyler, 374, 9-25.
    View publication on PubMed
Search for more literature for 1.14.16.5

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1.0 mM, 68% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
2-(1-octadecylthio)ethanol reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
3-(1-octadecylthio)propan-1,2-diol 0.5 mM, 64% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
8-hydroxyquinoline 1.0 mM, 15% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin; reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
8-Hydroxyquinoline-5-sulfonic acid 1.0 mM, 13% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
CHAPS 0.2% or 0.5%, activity is reduced by ca. 15% after 30 min Rattus norvegicus
EDTA 5 mM, 16% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
hexadecan-1,2-diol reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
NEM 8.0 mM; 91% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
octadecan-1-ol 0.1 mM, 15% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin Rattus norvegicus
Triton X-100 6% loss of activity at 0.1% detergent and 59% loss of activity at 0.3% detergent Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus
0.011
-
 RS-2-(1-hexadecyloxy)propan-1,2-diol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.0183
-
 2-hexadecyloxypropane-1,3-diol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.0204
-
 S-3-octadecyloxypropane-1,2-diol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.0246
-
 6R-5,6,7,8-tetrahydrobiopterin reaction with batyl alcohol Rattus norvegicus
0.0247
-
 RS-3-octadecyloxypropane-1,2-diol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.0265
-
 RS-3-(1-hexadecyloxy)1-methoxypropan-2-ol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.0313
-
 2-octadecyloxyethan-1-ol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.0397
-
 R-3-pentadecyloxypropane-1,2-diol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.0415
-
 R-3-octadecyloxypropane-1,2-diol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.1
-
 RS-3-(1-hexadecyloxy)propan-1-ol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.137
-
 S-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
0.173
-
 RS-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus
51.3
-
 RS-3-heptyloxypropane-1,2-diol with 6-methyl-5,6,7,8-tetrahydropterin as cofactor Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(RS)-batyl alcohol + 6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
2-(1-hexadecyloxy)propan-1,3-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
2-(1-octadecyloxy)ethanol + RS-(cis)-6,7-dimethyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
2-(1-octadecyloxy)ethanol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
3-(1-octadecylthio)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
batyl alcohol + 6R-5,6,7,8-tetrahydrobiopterin + O2 i.e. RS-3-(1-octadecyloxy)propan-1,2-doiol Rattus norvegicus ?
-
 ?
batyl alcohol + RS-(cis)-6,7-dimethyl-5,6,7,8-tetrahydropterin + O2 i.e. RS-3-(1-octadecyloxy)propan-1,2-diol Rattus norvegicus ?
-
 ?
R-3-(1-octadecyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
R-3-pentadecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
RS-3-(1-heptyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin
-
 Rattus norvegicus ?
-
 ?
RS-3-(1-hexadecyloxy)-1-methoxypropan-2-ol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
RS-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
RS-3-(1-hexadecyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
RS-3-(1-hexadecyloxy)propan-1-ol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
RS-3-(1-oleyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
S-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
S-3-(1-octadecyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 formation of glyceryl ether products Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
(6R)-5,6,7,8-tetrahydrobiopterin
-
 Rattus norvegicus
(RS)-6,7-dimethyl-5,6,7,8-tetrahydropterin
-
 Rattus norvegicus
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
 Rattus norvegicus