Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.4 extracted from

  • Friedman, P.A.; Kappelman, A.H.; Kaufman, S.
    Partial purification and characterization of tryptophan hydroxylase from rabbit hindbrain (1972), J. Biol. Chem., 247, 4165-4173.
    View publication on PubMed
Search for more literature for 1.14.16.4

General Stability

General Stability Organism
Fe2+ protects against inactivation by H2O2 in absence of catalase Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
H2O2 complete dependence on catalase in the absence of a mercaptan Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 20% O2, cofactor 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine, 2.5% O2, cofactor tetrahydrobipterine Oryctolagus cuniculus
0.031
-
 tetrahydrobiopterin
-
 Oryctolagus cuniculus
0.05
-
 tryptophan cofactor tetrahydrobiopterine Oryctolagus cuniculus
0.078
-
 tryptophan cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Oryctolagus cuniculus
0.13
-
 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine
-
 Oryctolagus cuniculus
0.29
-
 tryptophan cofactor 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulates in the absence of catalase Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
brain hindbrain Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00018
-
 partially purified enzyme from hindbrain Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-80°C, N2 atmosphere, dithiothreitol, 2 weeks, 20% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine + O2
-
 Oryctolagus cuniculus ?
-
 r
L-tryptophan + tetrahydrobiopterin + O2
-
 Oryctolagus cuniculus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
L-tryptophan + tetrahydropteridine + O2 cofactor tetrahydrobiopterin Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?